Hematopoietic stem cell and marrow stromal cell for spinal cord injury in mice
Hematopoietic stem cell and marrow stromal cell for spinal cord injury in mice
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DOI：
10.1097/01.wnr.0000183329.05994.d7
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发表时间：
2005-11-07
期刊：
影响因子：
1.7
通讯作者：
Yamazaki, M
中科院分区：
文献类型：
--
作者：
Koda, M;Okada, S;Yamazaki, M
我们比较了造血干细胞和骨髓基质细胞移植对小鼠脊髓损伤的影响。从绿色荧光蛋白转基因小鼠骨髓中，将谱系阴性、c-kit 和 Sca-1 阳性细胞分类为造血干细胞，并将塑料贴壁细胞培养为骨髓基质细胞。损伤后1周，将造血干细胞或骨髓基质细胞注射到病变部位。评估功能恢复并进行免疫组织化学分析。在造血干细胞组中，部分绿色荧光蛋白阳性细胞表达胶质标记。骨髓干细胞组中观察到大量绿色荧光蛋白和纤连蛋白双阳性细胞。两组之间的恢复情况没有观察到显着差异。造血干细胞和骨髓基质细胞都具有修复受损脊髓并促进功能恢复的潜力。
We compared the effects of hematopoietic stem cell and marrow stromal cell transplantation for spinal cord injury in mice. From green fluorescent protein transgenic mouse bone marrow, lineage-negative, c-kit- and Sca-1-positive cells were sorted as hematopoietic stem cells and plastic-adherent cells were cultured as marrow stromal cells. One week after injury, hematopoietic stem cells or marrow stromal cells were injected into the lesioned site. Functional recovery was assessed and immunohistochemistry was performed. In the hematopoietic stem cell group, a portion of green fluorescent protein-positive cells expressed glial marker. In the marrow stem cell group, a number of green fluorescent protein and fibronectin-double positive cells were observed. No significant difference was observed in the recovery between both groups. Both hematopoietic stem cells and marrow stromal cells have the potential to restore the injured spinal cord and to promote functional recovery.