A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)
A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)
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DOI：
10.1016/j.virol.2016.06.013
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发表时间：
2016-09-01
期刊：
影响因子：
3.7
通讯作者：
Delwart, Eric
中科院分区：
文献类型：
--
作者：
Phan, Tung G.;Dreno, Brigitte;Delwart, Eric
我们从腹泻儿童粪便中提取了原细小病毒属的7个几乎完整的基因组。这些病毒暂时被命名为cutaviruses（CutaV），其核苷酸变异为1-6%，与它们最近的亲戚人蟾蜍病毒的NS 1和VP 1具有相似的76%和相似的82%的氨基酸同源性。用PCR方法检测巴西和博茨瓦纳腹泻标本中的角质病毒DNA，检出率分别为1.6%（4/245）和1%（1/100）。对现有宏基因组数据集的计算机分析揭示了嗜表皮性皮肤T细胞淋巴瘤（CTCL或蕈样肉芽肿）患者皮肤活检中密切相关的细小病毒基因组。皮肤活检的PCR在4/17例CTCL、0/10例皮肤癌和0/21例正常或非癌皮肤活检中检出角质病毒DNA。CTCL皮肤活检的原位杂交检测到肿瘤浸润区域中罕见的单个细胞内的病毒基因组。cutavirus感染对人类肠道功能的影响和CTCL进展中可能的溶瘤作用仍有待确定。(C)2016 Elsevier Inc. All rights reserved.
We genetically characterized seven nearly complete genomes in the protoparvovirus genus from the feces of children with diarrhea. The viruses, provisionally named cutaviruses (CutaV), varied by 1-6% nucleotides and shared similar to 76% and similar to 82% amino acid identity with the NS1 and VP1 of human bufaviruses, their closest relatives. Using PCR, cutavirus DNA was found in 1.6% (4/245) and 1% (1/100) of diarrhea samples from Brazil and Botswana respectively. In silico analysis of pre-existing metagenomics datasets then revealed closely related parvovirus genomes in skin biopsies from patients with epidermotropic cutaneous T-cell lymphoma (CTCL or mycosis fungoides). PCR of skin biopsies yielded cutavirus DNA in 4/17 CTCL, 0/10 skin carcinoma, and 0/21 normal or noncancerous skin biopsies. In situ hybridization of CTCL skin biopsies detected viral genome within rare individual cells in regions of neoplastic infiltrations. The influence of cutavirus infection on human enteric functions and possible oncolytic role in CTCL progression remain to be determined. (C) 2016 Elsevier Inc. All rights reserved.