EXCITATORY AMINO-ACID RECEPTOR SUBTYPE BINDING FOLLOWING TRAUMATIC BRAIN INJURY
EXCITATORY AMINO-ACID RECEPTOR SUBTYPE BINDING FOLLOWING TRAUMATIC BRAIN INJURY
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DOI：
10.1016/0006-8993(90)90254-9
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发表时间：
1990-08-27
期刊：
影响因子：
2.9
通讯作者：
HAYES, RL
中科院分区：
文献类型：
--
作者：
MILLER, LP;LYETH, BG;HAYES, RL
使用液体冲击使 Sprague-Dawley 大鼠遭受中度（2.2 atm）的创伤性脑损伤（TBI）。受伤动物的创伤后存活期为 5 分钟、3 和 24 小时。通过定量放射自显影术评估每组中 N-甲基-D-天冬氨酸 (NMDA)、使君子氨酸和红藻氨酸受体亚群的区域谷氨酸受体亚型结合在约 -3.8 前囟水平，并与假手术对照组进行比较。 TBI 后 3 小时，海马 CA1 放射层、齿状回分子层以及覆盖新皮质的外层（第 1-3 层）和内层（第 5 层和第 6 层）中，[3H]谷氨酸与 NMDA 受体的结合显着降低（P < 0.05）。在所有创伤后存活时间内，新皮质第 5 层和第 6 层的 NMDA 受体结合显着减少，但在海马中没有看到进一步的差异。 TBI 后 24 小时，[3H]AMPA 与使君子酸受体的结合没有观察到显着变化，[3H]KA 结合仅在新皮质的第 5 层和第 6 层显着减少。这些数据进一步证实了该模型中选择性易受中等水平 TBI 影响的大脑区域中 NMDA 受体复合体的病理学参与。
Sprague-Dawley rats were subjected to a moderate level (2.2 atm) of traumatic brain injury (TBI) using fluid percussion. Injured animals were allowed post-trauma survival periods of 5 min, 3 and 24 h. Regional glutamate receptor subtype binding was assessed with quantitative autoradiography in each group for N-methyl-D-aspartate (NMDA), quisqualate and kainate receptor subpopulations at approximately the -3.8 bregma level and compared to a sham control group. [3H]glutamate binding to the NMDA receptor was significantly (P < 0.05) decreased at 3 h post-TBI in the hippocampal CA1 stratum radiatum, the molecular layers of the dentate gyri and the outer (layers 1-3) and inner (layers 5 and 6) overlying neocortex. NMDA receptor binding was significantly reduced in layers 5 and 6 of the neocortex at all post-trauma survival times but no further differences were seen in the hippocampi. No significant changes were observed with [3H]AMPA binding to quisqualate receptors and [3H]KA biding was significantly reduced only in layers 5 and 6 of the neocortex at 24 h after TBI. These data further confirm the pathological involvement of the NMDA recpetor complex in brain regions selectively vulnerable to moderate levels of TBI in this model.