Construction of infectious cDNA clones derived from the potyviruses Clover yellow vein virus and Bean yellow mosaic virus
Construction of infectious cDNA clones derived from the potyviruses Clover yellow vein virus and Bean yellow mosaic virus
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马铃薯病毒属三叶草黄脉病毒和豆黄花叶病毒感染性 cDNA 克隆的构建
DOI：
10.1007/978-1-4939-1743-3_16
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发表时间：
2015
影响因子：
--
通讯作者：
Ichiro Uyeda
中科院分区：
文献类型：
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作者：
Kenji S. Nakahara;Kei Nishino;Ichiro Uyeda
感染性cDNA克隆是研究病毒毒力和宿主抗性相关因子的重要工具。此外，在受感染植物中表达外源基因的感染性cDNA衍生的病毒载体使得能够以低成本生产有用的蛋白质，并且可以赋予新的作物性状。我们构建了两种马铃薯Y病毒属病毒的感染性cDNA克隆，即三叶草黄脉病毒和菜豆黄花叶病毒，这两种病毒感染豆科植物并引起病害。在这里，我们提出了我们的程序构建这些马铃薯Y病毒感染性克隆。
Infectious cDNA clones are now indispensible tools for the genetic analysis of viral factors involved in viral virulence and host resistance. In addition, infectious cDNA-derived virus vectors that express foreign genes in infected plants enable the production of useful proteins at low cost and can confer novel crop traits. We constructed infectious cDNA clones derived from two potyviruses,Clover yellow vein virusandBean yellow mosaic virus, which infect legume plants and cause disease. Here, we present our procedure for constructing these potyvirus infectious clones.