Increasing fidelity and efficiency by modifying cytidine base-editing systems in rice
Increasing fidelity and efficiency by modifying cytidine base-editing systems in rice
复制标题
通过修改水稻胞苷碱基编辑系统来提高保真度和效率
DOI：
10.1016/j.cj.2019.04.007
复制
发表时间：
2020-06-01
期刊：
影响因子：
6.6
通讯作者：
Wei, Pengcheng
中科院分区：
文献类型：
--
作者：
Qin, Ruiying;Liao, Shengxiang;Wei, Pengcheng
植物胞苷碱基编辑系统的效率是有限的，而且转基因植物中经常会发生不想要的突变。我们通过共表达三个拷贝的游离尿嘧啶-DNA糖基(UDG)抑制物(UGI)，提高了植物碱基编辑者3(BE3)和靶向激活诱导胞苷脱氨酶(CDA)(靶向-AID)系统的胞苷编辑频率和保真度。改良的BE3和CDA系统在再生水稻植株上的编辑效率分别高达88.9%和85.7%，而且不想要的突变频率很低。修改后的CDA系统可以克服BE3系统在GC环境中编辑频率低的问题。这些结果为水稻基因组碱基编辑提供了一种高保真、高效的解决方案。(C)2019年中国作物科学学会和中国科学院作物科学研究所。爱思唯尔B.V.代表科爱通信有限公司制作和主办。
The efficiency of plant cytidine base-editing systems is limited, and unwanted mutations frequently occur in transgenic plants. We increased the cytidine editing frequency and fidelity of the plant base editor 3 (BE3) and targeted activation-induced cytidine deaminase (CDA) (target-AID) systems by coexpressing three copies of free uracil-DNA glycosylase (UDG) inhibitor (UGI). The editing efficiency of the improved BE3 and CDA systems reached as high as 88.9% and 85.7%, respectively, in regenerated rice plants, with a very low frequency of unwanted mutations. The low editing frequency of the BE3 system in the GC context could be overcome by the modified CDA system. These results provide a high-fidelity and high-efficiency solution for rice genomic base editing. (C) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.