Should we ban total phenolics and antioxidant screening methods? The link between antioxidant potential and activation of NF-κB using phenolic compounds from grape by-products
Should we ban total phenolics and antioxidant screening methods? The link between antioxidant potential and activation of NF-κB using phenolic compounds from grape by-products
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DOI：
10.1016/j.foodchem.2019.03.145
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发表时间：
2019-08-30
期刊：
影响因子：
8.8
通讯作者：
Shahidi, Fereidoon
中科院分区：
文献类型：
--
作者：
de Camargo, Adriano Costa;Telles Biasoto, Aline Camarao;Shahidi, Fereidoon
自由基失衡与几种慢性疾病有关。然而，最近的争论已经检查了比色法筛选多酚功能的有效性。因此，在本研究中，基于化学反应的两种抗氧化剂方法被测试了其使用LPS激活的RAW 264.7巨噬细胞（被选择作为生物模型）预测NF-κ 3激活减少的能力。葡萄酒加工副产物的总酚含量、抗氧化能力（31.1%）和还原力（39.5%）均高于葡萄汁副产物。当通过减少活化NF-κ B针对LPS活化的RAW 264.7巨噬细胞测试这些样品时，观察到相同的趋势。含有较高TPC和相应ORAC和FRAP结果的原料转化为NF-κ 3活化的较高降低（36.5%）。因此，这一贡献表明，比色法仍然是重要的筛选工具，由于其简单和广泛的应用。
Free radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their ability in anticipating the reduction of the activation of NF-kappa 3 using LPS-activated RAW 264.7 macrophages, selected as a biological model. Grape processing by-products from winemaking showed higher total phenolic content (TPC), antioxidant capacity towards peroxyl radical (31.1%) as well as reducing power (39.5%) than those of grape juice by-products. The same trend was observed when these samples were tested against LPS-activated RAW 264.7 macrophages by reducing the activation NF-kappa B. Feedstocks containing higher TPC and corresponding ORAC and FRAP results translated to higher reduction in the activation of NF-kappa 3 (36.5%). Therefore, this contribution demonstrates that colorimetric methods are still important screening tools owing their simplicity and widespread application.