Cypermethrin-induced oxidative stress in rat brain and liver is prevented by Vitamin E or allopurinol
Cypermethrin-induced oxidative stress in rat brain and liver is prevented by Vitamin E or allopurinol
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DOI：
10.1016/s0378-4274(00)00277-0
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发表时间：
2001-01-03
影响因子：
3.5
通讯作者：
Hincal, F
中科院分区：
文献类型：
--
作者：
Giray, B;Gürbay, A;Hincal, F
考虑到活性氧（ROS）与各种农药的毒性有关，本研究旨在探讨氯氰菊酯（一种II型拟除虫菊酯）诱导氧化应激的可能性。单次（170 mg/kg）或重复（每天 75 mg/kg，持续 5 天）口服氯氰菊酯被发现会在大鼠的脑和肝组织中产生显着的氧化应激，治疗后 4 或 24 小时，两个组织中硫代巴比妥酸反应物质 (TBARS) 水平的升高就证明了这一点。在肝脏中观察到更高的变化，单剂量从 4 小时时的 60% 增加到 24 小时对照的近 4 倍。总谷胱甘肽（总 GSH）水平降低（高达 20%）和共轭二烯升高（类似于 4 小时单剂量肝脏中的 60%）也表明存在氧化损伤。然而，脑组织和肝组织中任何剂量或所有时间点的谷胱甘肽-S-转移酶（GST）活性与对照值没有差异。用别嘌呤醇（100 mg/kg，腹腔注射）或维生素 E（每天 100 mg/kg，注射，持续 3 天，第 4 天剂量 40 mg/kg）预处理大鼠，可显着防止 4 小时内单次口服高剂量氯氰菊酯引起的脑和肝组织中 TBARS 水平升高。因此，结果表明，大鼠接触氯氰菊酯会导致自由基介导的组织损伤，表现为脑和肝脂质过氧化升高，而别嘌呤醇和维生素 E 可以防止这种情况的发生。(C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
Considering that the involvement of reactive oxygen species (ROS) has been implicated in the toxicity of various pesticides, this study was designed to investigate the possibility of oxidative stress induction by cypermethrin, a Type II pyrethroid. Either single (170 mg/kg) or repeated (75 mg/kg per day for 5 days) oral administration of cypermethrin was found to produce significant oxidative stress in cerebral and hepatic tissues of rats, as was evident by the elevation of the level of thiobarbituric acid reactive substances (TBARS) in both tissues, either 4 or 24 h after treatment. Much higher changes were observed in liver, increasing from a level of 60% at 4 h up to nearly 4 times the control at 24 h for single dose. Reduced levels (up to 20%) of total glutathione (total GSH), and elevation of conjugated dienes ( similar to 60% in liver by single dose at 4 h) also indicated the presence of an oxidative insult. Glutathione-S-transferase (GST) activity, however, did not differ from control values for any dose or at ally time point in cerebral and hepatic tissues. Pretreatment of rats with allopurinol (100 mg/kg, ip) or Vitamin E (100 mg/kg per day, ig, for 3 days and a dose of 40 mg/kg on the 4th day) provided significant protection against the elevation of TBARS levels in cerebral and hepatic tissues, induced by single high dose of oral cypermethrin administration within 4 h. Thus, the results suggest that cypermethrin exposure of rats results in free radical-mediated tissue damage, as indicated by elevated cerebral and hepatic lipid peroxidation, which was prevented by allopurinol and Vitamin E. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.