Different cytokine profiles of intrahepatic T cells in chronic hepatitis B and hepatitis C virus infections
Different cytokine profiles of intrahepatic T cells in chronic hepatitis B and hepatitis C virus infections
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DOI：
10.1016/s0016-5085(97)70235-x
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发表时间：
1997-01-01
期刊：
影响因子：
29.4
通讯作者：
Ferrari, C
中科院分区：
文献类型：
--
作者：
Bertoletti, A;DElios, MM;Ferrari, C
背景和目标：T细胞在病毒复制部位分泌的细胞因子模式可能影响B型肝炎病毒（HBV）和丙型肝炎病毒（HCV）感染的最终结果。本研究的目的是评估是否有细胞因子失衡倾向于辅助性T细胞（Th）1或Th 2型应答可能在慢性B或丙型肝炎中发挥作用。研究方法：6例慢性B型肝炎患者肝脏来源的T细胞克隆产生IFN-γ、IL-4和IL-5的研究（291个克隆）和9例慢性肝炎6（260个克隆）进行了研究，T型通过有限稀释法从来自肝组织的新鲜分离的单核细胞中产生细胞克隆，以提供可靠的代表性。结果：慢性丙型肝炎患者肝脏浸润性T细胞主要为Th 1细胞，能分泌IFN-γ，但不能分泌IL-4或IL-5;而在B型肝炎患者，大多数CD 4（+）和CD 8（+）肝脏T细胞为ThO样细胞，不仅能分泌IFN-γ，还能分泌IL-4和IL-5。慢性HBV和HCV感染中肝脏内T细胞的不同细胞因子谱说明了这两种感染中局部免疫应答的不同行为，其可能具有致病性影响。
Background & Aims: The cytokine pattern secreted by T cells at the site of viral replication may influence the final outcome of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, The aim of this study was to assess whether a cytokine imbalance oriented toward T helper (Th) 1 or Th2-type responses may play a role in chronic hepatitis B or C. Methods: Production of interferon (IFN)-gamma, interleukin (IL)-4, and IL-5 by wide series of T-cell clones derived from the liver of 6 patients with chronic hepatitis B (291 clones) and 9 patients with chronic hepatitis 6 (260 clones) was studied, T-cell clones were generated by limiting dilution from freshly isolated mononuclear cells derived from liver tissue to give a reliable representation of the intrahepatic inflammatory infiltrates, Results: The majority of liver-infiltrating T cells in chronic hepatitis C were Th1 cells able to secrete IFN-gamma but unable to secrete IL-4 or IL-5, whereas in hepatitis B, most CD4(+) and CD8(+) liver T cells were ThO-like cells able to produce not only IFN-gamma but also IL-4 and IL-5, Conclusions: The different cytokine profiles of T cells within the liver in chronic HBV and HCV infections illustrate a different behavior of the local immune response in these two infections that may have pathogenetic implications.