Molecular diversity of bacteria in Yunnan yellow cattle (Bos taurs) from Nujiang region, China
Molecular diversity of bacteria in Yunnan yellow cattle (Bos taurs) from Nujiang region, China
复制标题
怒江地区云南黄牛细菌的分子多样性
DOI：
10.1007/s11033-011-0848-5
复制
发表时间：
2012-02-01
影响因子：
2.8
通讯作者：
Mao, H. M.
中科院分区：
文献类型：
--
作者：
Leng, J.;Cheng, Y. M.;Mao, H. M.
采集4头云南黄牛瘤胃内容物，通过16S rRNA基因序列分析测定细菌多样性。总共检查了129个序列，根据基因序列相似度为97%，将这些序列称为107个OTU(Operational Taxonomy Unit)。相似性分析显示，云南黄牛有12个序列（10个OTU）与培养瘤胃细菌溶纤维丁酸弧菌、瘤胃琥珀酸、布氏瘤胃球菌、破破烂梭菌、黄色瘤胃球菌、瘤胃假丁酸弧菌、嗜冷嗜冷球菌、和Prevotella ruminicola，占克隆总数的9.3%（占总OTU的9.2%）。另外12个序列（9个OTU）与培养细菌氨基丁酸梭菌、产丁酸菌、Schwartzia succinivorans、Prevotella ruminicola、Eubacterum ruminantium、Ruminococcus albus和Clostridium termitidis具有90-97%的相似性，也占总序列的9.3%（占总OTU的8.3%）。其余105条序列（90个OTU）与培养细菌的相似度低于90%，占总序列的81.4%（占总OTU的82.5%）。根据系统发育分析，所有序列均属于低G+C亚门（分别占克隆总数和OTU的72.1%和72.5%）和CFB亚门（噬细胞菌-柔韧杆菌-拟杆菌；分别占克隆总数和OTU的27.9%和27.5%）。在检查的克隆中，在牛的瘤胃中检测到了稀有细菌嗜冷菌。
The rumen content of four Yunnan Yellow Cattle (Bos taurs) were collected to determine the bacteria diversity by using 16S rRNA gene sequence analysis. A total of 129 sequences were examined and the sequences were referred as 107 OTU (Operational Taxonomy Unit) according to the similarity level of 97% in gene sequence. Similarity analysis revealed that Yunnan Yellow Cattle had 12 sequences (10 OTU) shared 97% or greater similarity with cultured rumen bacteriaButyrivibrio fibrisolvens,Succiniclasticum ruminis,Ruminococcus bromii,Clostridium proteoclasticum,Ruminococcus flavefaciens,Pseudobutyrivibrio ruminis,Jeotgalicoccus psychrophilus, andPrevotella ruminicola, which accounting for 9.3% of the total clones (9.2% of the total OTU). The further 12 sequences (9 OTU) shared 90–97% similarity with cultured bacteriaClostridium aminobutyricum,butyrate-producing bacterium,Schwartzia succinivorans,Prevotella ruminicola,Eubacterium ruminantium,Ruminococcus albus, andClostridium termitidis, also accounting for 9.3% of the total sequences (8.3% of the total OTU). The remaining 105 sequences (90 OTU) shared less than 90% similarity with cultured bacteria, accounting for 81.4% of the total sequences (82.5% of the total OTU). According to the phylogenetic analysis, all sequences were phylogenetically placed within phyla of low G+C subdivision (accounting for 72.1 and 72.5% of the total clones and OTU, respectively) and CFB subdivision (Cytophaga-Flexibacter-Bacteroides; accounting for 27.9 and 27.5% of the total clones and OTU, respectively). Among the examined clones, rare bacteriaJeotgalicoccus psychrophiluswas detected in the rumen of cattle.