Fractionation studies of selenium compounds from oysters, and their determination by high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry
Fractionation studies of selenium compounds from oysters, and their determination by high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry
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牡蛎中硒化合物的分级研究及其高效液相色谱-电感耦合等离子体质谱法测定
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
C. Cámara
中科院分区：
文献类型：
--
作者：
P. Moreno;M. Quijano;A. Gutiérrez;M. Pérez;C. Cámara
本文介绍了一种水萃取分离牡蛎样品中硒的方法。检测了几个参数，以评价水浸提的效率：所用溶剂的性质、浓度、pH值和温度。水溶剂中的可溶部分含有总硒含量的35 ± 3%（1.22 ± 0.03 µg g−1）。随后的酶水解的液体提取物和固体残留物允许测定硒物种的高效液相色谱法结合电感耦合等离子体质谱法。在两种色谱模式（阴离子和阳离子交换）进行色谱分析，用于鉴定样品中的硒物种。在拟议处理下，牡蛎组织中发现的物质为三甲基硒离子（9.8 ± 0.8%）
硒代蛋氨酸（46 ± 6%）。通过使用质量截止过滤器，可以确定以分子量高于10 kDa的化学形式保留的硒的百分比，因此得出结论，酶促水解在可溶性部分中比在不溶性部分中更有效。
A procedure is described for the fractionation of selenium species in oyster samples by aqueous extraction. Several parameters were tested in order to evaluate the efficiency of the aqueous extraction: nature, concentration, pH and temperature of the solvent used. The soluble fraction in the aqueous solvent contains 35 ± 3% of the total selenium content (1.22 ± 0.03 µg g−1). The subsequent enzymatic hydrolysis of both the liquid extract and the solid residue allows the determination of selenium species by high-performance liquid chromatography in conjunction with inductively coupled plasma mass spectrometry. The chromatographic analyses were carried out in two chromatographic modes (anionic and cationic exchange) for the identification of selenium species in the samples. The species found in oyster tissues under the proposed treatment were trimethylselenonium ion (9.8 ± 0.8%)
and selenomethionine (46 ± 6%). By using mass cut-off filters it was possible to determine the percentage of selenium remaining in chemical forms with a molecular weight higher than 10 kDa, and therefore to conclude that enzymatic hydrolysis is more efficient in the soluble fraction than in the non-soluble one.
DOI：
10.1039/a907847i
发表时间：
2000
期刊：
The Analyst
影响因子：
--
作者：
K. Sutton;C. A. Ponce de León;K. L. Ackley;R. Sutton;A. Stalcup;J. Caruso
通讯作者：
K. Sutton;C. A. Ponce de León;K. L. Ackley;R. Sutton;A. Stalcup;J. Caruso
影响因子：
4.2
作者：
Kotrebai, M;Birringer, M;Uden, PC
通讯作者：
Uden, PC