Confocal mapping of cortical inputs onto identified pyramidal neurons
Confocal mapping of cortical inputs onto identified pyramidal neurons
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DOI：
10.2741/3159
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发表时间：
2008-05-01
影响因子：
3.1
通讯作者：
Rockland, Kathleen S.
中科院分区：
文献类型：
--
作者：
Ichinohe, Noritaka;Hyde, James;Rockland, Kathleen S.
使用共焦显微镜协议，我们对猴子颞叶皮层的皮层连接进行了微电路研究。通过在 TE 后区注射 BDA 来标记输入，并通过注射逆行运输的腺病毒来用 EGFP 标记潜在的突触后锥体神经元目标。我们对不同层神经元树突区室的假定接触的数量和分布进行了评分。初步结果表明，当层 (L.) 6 神经元上升穿过 L.4（n = 1 脑）时，约 50% 的顶端树突接收到接触，但只有 30-35% 来自 L.5 神经元（n = 2）。 L.3 神经元的基底树突在 L.4 中也几乎没有接触。这支持了第 4 层作为联合皮层层间中继的作用。此外，我们的结果表明接触的发生和数量存在空间异质性，这可能是由于目标偏好的亚型特异性所致。从前部 TE 投射到后部 TE 的 L.2 神经元的最大接触数量为 29。这种方法似乎是长距离连接电子显微镜分析的有用替代或补充。
Using a confocal microscopy protocol, we carried out a microcircuitry investigation of cortical connections in monkey temporal cortex. Inputs were labeled by BDA injections in posterior area TE, and potential postsynaptic pyramidal neuron targets were labeled with EGFP, by injection of retrogradely transported adenovirus. We scored the number and distribution of putative contacts onto dendritic compartments of neurons in different layers. Initial results show that about 50% of apical dendrites of layer (L.) 6 neurons receive contacts, as they ascend through L.4 (n= 1 brain), but only 30-35% of those from L.5 neurons (n= 2). Basal dendrites of L. 3 neurons also receive few contacts in L.4. This supports the role of layer 4 as an interlaminar relay in association cortex. In addition, our results indicate spatial heterogeneity in the occurrence and number of contacts, possibly due to subtype specificity in target preference. The maximum number of contacts, for a L. 2 neuron projecting from anterior to posterior TE, was 29. This approach seems a useful alternative or complement to electron microscopic analyses of long distance connectivity.