The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae
The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae
复制标题
DOI：
10.1111/tpj.14363
复制
发表时间：
2019-06-01
期刊：
影响因子：
7.2
通讯作者：
Borisjuk, Ljudmilla
中科院分区：
文献类型：
--
作者：
Radchuk, Volodymyr;Sharma, Rajiv;Borisjuk, Ljudmilla
系统发育相关的物种群包含谱系特有的基因，这些基因与谱系外的任何基因都没有序列相似性。我们在这里描述了有性繁殖所需的Jekyll基因存在于两个差异很大的等位基因变体Jek1和Jek3中。尽管相似性很低，但Jek1和Jek3蛋白具有相同的信号肽、保守的半胱氨酸位置和直接重复序列。Jek1/Jek3序列位于相同的染色体上，并以单基因孟德尔方式遗传。Jek3与Jek1有相似的表达，在Jek1缺失的植物中补充了Jek1的功能。Jek1和Jek3等位基因变异体在485份野生大麦和驯化大麦中的分布几乎相等。所有携带Jek1等位基因的驯化大麦都属于单一单倍型J1-H1，表明驯化过程中存在遗传瓶颈。携带Jek3等位基因的驯化大麦由三种单倍型组成。Jekyll类序列仅在关系密切的雀麦属和小麦族物种中发现，而在其他禾本科植物中未发现。非侵入性磁共振成像揭示了小麦亚科和雀麦亚科中与Jekyll功能相关的内在颗粒结构。Jekyll的出现表明了它在禾本科雀麦属和小麦族谱系分离中的作用，并确定Jekyll基因是谱系特有的。
Phylogenetically related groups of species contain lineage-specific genes that exhibit no sequence similarity to any genes outside the lineage. We describe here that the Jekyll gene, required for sexual reproduction, exists in two much diverged allelic variants, Jek1 and Jek3. Despite low similarity, the Jek1 and Jek3 proteins share identical signal peptides, conserved cysteine positions and direct repeats. The Jek1/Jek3 sequences are located at the same chromosomal locus and inherited in a monogenic Mendelian fashion. Jek3 has a similar expression as Jek1 and complements the Jek1 function in Jek1-deficient plants. Jek1 and Jek3 allelic variants were almost equally distributed in a collection of 485 wild and domesticated barley accessions. All domesticated barleys harboring the Jek1 allele belong to single haplotype J1-H1 indicating a genetic bottleneck during domestication. Domesticated barleys harboring the Jek3 allele consisted of three haplotypes. Jekyll-like sequences were found only in species of the closely related tribes Bromeae and Triticeae but not in other Poaceae. Non-invasive magnetic resonance imaging revealed intrinsic grain structure in Triticeae and Bromeae, associated with the Jekyll function. The emergence of Jekyll suggests its role in the separation of the Bromeae and Triticeae lineages within the Poaceae and identifies the Jekyll genes as lineage-specific.