Nanowire-based single-cell endoscopy
Nanowire-based single-cell endoscopy
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DOI：
10.1038/nnano.2011.226
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发表时间：
2012-03-01
影响因子：
38.3
通讯作者：
Yang, Peidong
中科院分区：
文献类型：
--
作者：
Yan, Ruoxue;Park, Ji-Ho;Yang, Peidong
基于纳米线和纳米管的一维智能探针可以安全地穿透质膜并进入生物细胞，在高分辨率（1-6）和高通量（7,8）基因和药物传递、生物传感（6,9）和单细胞电生理学（6,10）方面具有潜在的用途。然而，将这种探针用于亚波长水平的跨细胞膜光通信仍然是有限的。在这里，我们展示了附着在光纤锥形尖端的纳米线波导可以将可见光引导到活的哺乳动物细胞的细胞内，并且还可以以高空间分辨率检测来自亚细胞区域的光信号。此外，我们表明，通过光激活机制，内窥镜可以将有效载荷传递到具有空间和时间特异性的细胞中。此外，将内窥镜插入细胞和引导激光照射对细胞没有任何明显的毒性。
One-dimensional smart probes based on nanowires and nanotubes that can safely penetrate the plasma membrane and enter biological cells are potentially useful in high-resolution(1-6) and high-throughput(7,8) gene and drug delivery, biosensing(6,9) and single-cell electrophysiology(6,10). However, using such probes for optical communication across the cellular membrane at the subwavelength level remains limited. Here, we show that a nanowire waveguide attached to the tapered tip of an optical fibre can guide visible light into intracellular compartments of a living mammalian cell, and can also detect optical signals from subcellular regions with high spatial resolution. Furthermore, we show that through light-activated mechanisms the endoscope can deliver payloads into cells with spatial and temporal specificity. Moreover, insertion of the endoscope into cells and illumination of the guided laser did not induce any significant toxicity in the cells.