Optogenetic activation of the inhibitory nigro-collicular circuit evokes contralateral orienting movements in mice.
Optogenetic activation of the inhibitory nigro-collicular circuit evokes contralateral orienting movements in mice.
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DOI：
10.1016/j.celrep.2022.110699
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发表时间：
2022-04-19
期刊：
影响因子：
8.8
通讯作者：
Basso, Michele A.
中科院分区：
文献类型：
--
作者：
Villalobos, Claudio A.;Basso, Michele A.
我们报道，通过小鼠体内光遗传激活gaba能终端，基底节区（BG）通过黑质网状部（黑质）增加对上丘（SC）的抑制作用，产生对侧定向运动。这些运动是出乎意料的，因为神经活动的减少而不是增加通常与定向运动的开始有关。我们发现，在切片记录中，同样的光遗传刺激在体内产生运动的神经末梢引起抑制后反弹去极化，随后在SC输出神经元中出现Na+峰值。此外，在切片中阻断t型Ca2+通道可以防止SC输出神经元的抑制后反弹和随后的Na+峰值，并降低体内对侧定向的可能性。基于这些结果，我们提出，除了允许作用外，BG可能通过驱动SC输出神经元的抑制后反弹去极化，在小鼠定向运动的产生中发挥积极作用。Villalobos和Basso表明，除了已知的来自被认为抑制运动的黑丘回路的抑制外，小鼠黑质网状部向上丘的抑制性输入的光遗传激活唤起了对侧定向运动和抑制后丘输出神经元的反跳去极化。
We report that increasing inhibition from the basal ganglia (BG) to the superior colliculus (SC) through the substantia nigra pars reticulata (nigra) using in vivo optogenetic activation of GABAergic terminals in mice produces contralateral orienting movements. These movements are unexpected because decreases, and not increases, in nigral activity are generally associated with the initiation of orienting movements. We found that, in slice recordings, the same optogenetic stimulation of nigral terminals producing movements in vivo evokes post-inhibitory rebound depolarization followed by Na+ spikes in SC output neurons. Moreover, blocking T-type Ca2+ channels in slices prevent post-inhibitory rebound and subsequent Na+ spiking in SC output neurons and also reduce the likelihood of contralateral orienting in vivo. On the basis of these results, we propose that, in addition to the permissive role, the BG may play an active role in the generation of orienting movements in mice by driving post-inhibitory rebound depolarization in SC output neurons. Villalobos and Basso show that, in addition to the known inhibition from the nigro-collicular circuit, thought to suppress movement, optogenetic activation of the inhibitory inputs to the superior colliculus from the substantia nigra pars reticulata in the mouse evokes contralateral orienting movements and post-inhibitory rebound depolarization in collicular output neurons.
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