Expression of a voltage-dependent potassium channel protein (Kv3.1) in the embryonic development of the auditory system.
Expression of a voltage-dependent potassium channel protein (Kv3.1) in the embryonic development of the auditory system.
复制标题
电压依赖性钾通道蛋白 (Kv3.1) 在听觉系统胚胎发育中的表达。
DOI：
10.1002/jnr.1124
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Morest,DK
中科院分区：
文献类型：
--
作者：
Zhou,X;Baier,C;Hossain,WA;Goldenson,M;Morest,DK
本研究追踪了从早期发育阶段直到接近孵化的耳蜗核、耳蜗前庭神经节和耳上皮的鸟类同源物中电压依赖性钾通道蛋白（Kv3.1）的发育。在Hamburger-汉密尔顿分期鸡胚胎上使用羧基末端（识别Kv3.1b剪接变体）和氨基末端（识别Kv3.1的任一形式）抗体进行免疫组织化学。在这些区域中，免疫染色的相对水平存在三个阶段。早期（E2-6），当前体细胞增殖、迁移并形成轴突时，当使用任一抗体时存在染色。在中期（E6-11），以毛细胞分化、树突生长和早期突触形成为标志，染色水平下降。在晚期（E11-19），当听觉功能开始时，染色迅速增加，特别是Kv3.1b。早期Kv3.1表达发生在神经元和毛细胞前体细胞分化或发挥功能之前。随后，在耳上皮中，纤毛中高水平的Kv3.1可能先于毛细胞功能的发作或与毛细胞功能的发作一致。在神经元中，其定位的一些特征与轴突生长和突触形成相关，其他特征与神经活动和功能的开始相关。J. Neurosci. Res. 65：24-37，2001.© 2001 Wiley利斯公司
The present study traces the development of a voltage‐dependent potassium channel protein (Kv3.1) in the avian homologue of the cochlear nucleus, in the cochleovestibular ganglion, and in the otic epithelium from early developmental stages until near hatching. Immunohistochemistry with antibodies to the carboxy terminus (recognizing the Kv3.1b splice variant) and to the amino terminus (recognizing either form of Kv3.1) was used on Hamburger‐Hamilton‐staged chicken embryos. There were three periods in the relative levels of immunostaining in these regions. Early (E2–6), when precursor cells proliferate, migrate, and form axons, there was staining when using either antibody. In the middle period (E6–11), marked by hair cell differentiation, dendritic growth, and early synapse formation, staining levels decreased. In the late period (E11–19), when auditory function begins, staining increased rapidly, especially for Kv3.1b. Early Kv3.1 expression occurs in neuronal and hair cell precursors before they differentiate or function. Later, in the otic epithelium, a high level of Kv3.1 in cilia may precede or coincide with the onset of hair cell function. In neurons, some features of its localization correlate with axon outgrowth and synapse formation, others with the onset of neural activity and function. J. Neurosci. Res. 65:24–37, 2001. © 2001 Wiley‐Liss, Inc.