RETINOIDS INHIBIT PROMOTER‐DEPENDENT PRENEOPLASTIC PROGRESSION IN MOUSE EPIDERMAL CELL LINES
RETINOIDS INHIBIT PROMOTER‐DEPENDENT PRENEOPLASTIC PROGRESSION IN MOUSE EPIDERMAL CELL LINES
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类维生素A抑制小鼠表皮细胞系中启动子依赖性的肿瘤前期进展
DOI：
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发表时间：
1981
影响因子：
5.2
通讯作者：
Gina Abruzzo
中科院分区：
文献类型：
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作者：
N. Colburn;S. Ozanne;U. Lichti;T. Ben;S. Yuspa;E. Wendel;Edward Jardini;Gina Abruzzo
我们最近描述了一种用于研究启动子依赖的癌前进展的细胞培养系统。1.2 JB6细胞系从小鼠的原代表皮培养中分离出来，发现JB6细胞对促肿瘤的佛波酯有反应，但对非促肿瘤的佛波酯没有反应，并且不可逆地诱导肿瘤细胞的表型，通过在0.33%琼脂中14天的集落形成来测量。其机制似乎涉及诱导新的表型，而不是选择先前存在的锚定非依赖细胞。3 JB6前体细胞是非致瘤的，而佛波酯诱导的锚定非依赖转化子是肿瘤形成(Colburn，提交)。因此，鉴于其快速和不可逆性，JB6细胞对佛波醇酯的这种锚定非依赖性反应似乎类似于体内晚期皮肤肿瘤的促进作用。4~5 Boutwell及其同事报道，在促进阶段，类维A酸类化合物在小鼠皮肤上应用TPA前一小时，可显著抑制肿瘤的产生。6，7本报告中描述的实验是为了(1)确定维甲酸类化合物在细胞培养中是否具有抑制晚期促进作用的活性；(2)使用维甲酸抑制作为确定肿瘤促进过程中所需的分子和细胞事件的方法，以及(3)研究维甲酸类化合物在肿瘤预防中的作用模式。
We have recently described a cell culture system for studying promoterdependent preneoplastic progression.l.2 The JB6 cell line has been derived from primary mouse epidermal cultures and found to respond to tumor-promoting but not to nonpromoting phorbol esters with an irreversible induction of tumor-cell phenotype, as measured by colony formation in 0.33% agar at 14 days. The mechanism appears to involve induction of a new phenotype rather than selection of preexisting anchorage-independent cells.3 The JB6 precursor cells are noatumorigenic while the phorbol ester-induced anchorage-independent transformants are tumorigenic (Colburn, submitted). Thus in view of its rapidity and irreversibility this anchorage-independence response to phorbol esters by JB6 cells appears to be analogous to late-stage skin tumor promotion in vivo. Inhibition of preneoplastic progression by retinoids has been shown for a number of epithelial systems.4~5 Boutwell and coworkers have reported that retinoids applied to mouse skin one hour before TPA during the promotion phase inhibited tumor yield substantially.6,7 The experiments described in this report were undertaken (1) to determine whether retinoids are active in inhibiting late-stage promotion in cell culture; (2) to use retinoid inhibition as an approach to identifying molecular and cellular events that are required in the process of tumor promotion, and (3) to investigate the mode of action of retinoids in tumor prophylaxis.
通过重复 12-o-十四烷酰佛波醇-13-乙酸酯处理来超诱导小鼠表皮鸟氨酸脱羧酶活性。
影响因子：
4.3
作者：
Verma,AK;Hsiao,KM;Ahrens,H;Suganuma,M;Fujiki,H;Matsufuji,S;Hayashi,H
通讯作者：
Hayashi,H