Molecular characterization of two pheromone binding proteins and quantitative analysis of their expression in the beet armyworm, Spodoptera exigua Hubner
Molecular characterization of two pheromone binding proteins and quantitative analysis of their expression in the beet armyworm, Spodoptera exigua Hubner
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DOI：
10.1007/s10886-007-9277-2
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发表时间：
2007-05-01
影响因子：
2.3
通讯作者：
Dong, Shuang-Lin
中科院分区：
文献类型：
--
作者：
Xiu, Wei-Ming;Dong, Shuang-Lin
信息素结合蛋白(PBP)在昆虫信息素通讯中起着重要作用。然而，甜菜夜蛾是一种世界性的重要农业害虫，对甜菜夜蛾的危害仍未得到解决。利用逆转录-聚合酶链式反应(RT-PCR)和快速扩增cDNAEnds-PCR(RACE-PCR)技术，从甜菜夜蛾触角DNA中克隆了PBP基因SexigPBP1和SexigPBP2。推导出的PBP氨基酸序列是气味结合蛋白(OBP)家族的特征，尽管两个PBP只有44%相同。基因组DNA分析表明，两个PBP基因各有两个内含子和相似的内含子/外显子结构模式。RT-PCR分析表明，这两个PBP基因仅在触角中表达。荧光定量聚合酶链式反应进一步表明，无论性别，SexigPBP1的表达水平都明显高于SexigPBP2。然而，女性SexigPBP1和SCxigPBP2的表达水平分别约为男性的39%和73%。最后，系统发育分析表明，根据初级序列，夜蛾科的多氯联苯可分为三个不同的组。
Pheromone binding proteins (PBP) play an important role in insect pheromone communication. However, the PBP for the beet armyworm, Spodoptera exigua Hubner (Lepidoptera: Noctuidae), an important agricultural pest worldwide, remains unaddressed. We report the cloning of two PBP genes, SexigPBP1 and SexigPBP2, from the antennal cDNA of S. exigua by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends-PCR (RACE-PCR). The deduced PBP amino acid sequences are characteristic of the odorant binding protein (OBP) family, although the two PBPs are only 44% identical. From an analysis of the genomic DNA, two introns and a similar intron/extron structural pattern were identified in each of the two PBP genes. RT-PCR analysis revealed that the two PBP genes are only expressed in antennae. Real-time PCR further indicated that the expression of SexigPBP1 is much higher than that of SexigPBP2, regardless of sex. However, the female expression levels for SexigPBP1 and SCxigPBP2 are about 39% and 73%, respectively, relative to male levels. Finally, phylogenetic analysis suggested that PBPs from the Noctuidae are divided into three distinct groups based on the primary sequences.