Extrachromosomal deer fibromavirus DNA in deer fibromas and virus-transformed mouse cells.
Extrachromosomal deer fibromavirus DNA in deer fibromas and virus-transformed mouse cells.
复制标题
鹿纤维瘤和病毒转化小鼠细胞中的染色体外鹿纤维瘤病毒 DNA。
DOI：
10.1016/0042-6822(83)90519-6
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发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Lancaster,WD
中科院分区：
文献类型：
--
作者：
Groff,DE;Sundberg,JP;Lancaster,WD
对5例鹿纤维瘤不产生病毒的纤维瘤部位进行了鹿纤维病毒(DFV)DNA序列检测。液相杂交显示病毒基因组的每个细胞有100到330个拷贝。对未消化的鹿肿瘤DNA样品的Southern杂交分析表明，大多数DFV DNA是以单体、未整合的基因组形式存在的；然而，限制性内切酶酶切图谱表明，有一小部分DFV序列具有抗性。在病毒转化的NIH/3T3小鼠细胞中，DFV DNA也以多个染色体外基因组的形式存在。
The non-virus-producing fibromatous portions of five deer fibromas were examined for deer fibromavirus (DFV) DNA sequences. Liquid-phase hybridization revealed 100 to 330 copies per cell of the virus genome. Southern blot analysis of undigested deer tumor DNA preparations indicated that most of the DFV DNA was present as monomeric, unintegrated genomes; however, restriction enzyme digestion patterns suggest a small population of resistant DFV sequences. DFV DNA was also present in virus-transformed NIH/3T3 mouse cells as multiple, extrachromosomal genomes.