Xenon improves neurologic outcome and reduces secondary injury following trauma in an in vivo model of traumatic brain injury.
Xenon improves neurologic outcome and reduces secondary injury following trauma in an in vivo model of traumatic brain injury.
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DOI：
10.1097/ccm.0000000000000624
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发表时间：
2015-01
影响因子：
8.8
通讯作者：
Dickinson R
中科院分区：
文献类型：
--
作者：
Campos-Pires R;Armstrong SP;Sebastiani A;Luh C;Gruss M;Radyushkin K;Hirnet T;Werner C;Engelhard K;Franks NP;Thal SC;Dickinson R
确定惰性气体氙气在创伤性脑损伤后的神经保护功效，并确定氙气的应用是否具有临床相关的治疗时间窗。对照动物研究。大学研究实验室。雄性 C57BL/6N 小鼠 (n=196) 在通过受控皮质冲击造成机械性脑损伤后接受 75% 氙气、50% 氙气或 30% 氙气加 25% 氧气（平衡氮）治疗。使用以下方法测量创伤后的结果：1）功能神经学结果评分，2）挫伤体积的组织学测量，3）运动功能和步态分析。我们的研究表明，氙气治疗可以改善创伤性脑损伤后的预后。在受伤后的早期（24 小时）和长达 4 天内，氙治疗组的神经学结果评分显着更好（p<0.05）。氙治疗组的挫伤体积显着减少（p<0.05）。当损伤后 15 分钟给予氙气或损伤后延迟 1 小时或 3 小时治疗时，氙气治疗显着 (p<0.05) 减少了挫伤体积。损伤后 15 分钟或 1 小时进行氙气治疗时，神经系统结果显着改善 (p<0.05)。创伤后 1 个月，氙治疗组观察到运动功能改善（p<0.05）。这些结果首次表明，氙可以改善小鼠脑外伤后的神经系统结果并减少挫伤体积。在此模型中，氙气应用的治疗时间窗至少长达 3 小时。这些发现支持这样的观点：氙可能有助于脑外伤患者的神经保护治疗。
To determine the neuroprotective efficacy of the inert gas xenon following traumatic brain injury, and to determine whether application of xenon has a clinically relevant therapeutic time window. Controlled animal study. University research laboratory. Male C57BL/6N mice (n=196) 75% xenon, 50% xenon or 30% xenon, with 25% oxygen (balance nitrogen) treatment following mechanical brain lesion by controlled cortical impact. Outcome following trauma was measured using: 1) functional neurological outcome score, 2) histological measurement of contusion volume, 3) analysis of locomotor function and gait. Our study shows that xenon-treatment improves outcome following traumatic brain injury. Neurological outcome scores were significantly (p<0.05) better in xenon-treated groups in the early phase (24 hours) and up to 4 days after injury. Contusion volume was significantly (p<0.05) reduced in the xenon-treated groups. Xenon treatment significantly (p<0.05) reduced contusion volume when xenon was given 15 minutes after injury or when treatment was delayed 1 hour or 3 hours after injury. Neurological outcome was significantly (p<0.05) improved when xenon treatment was given 15 minutes or 1 hour after injury. Improvements in locomotor function (p<0.05) were observed in the xenon-treated group, 1 month after trauma. These results show for the first time that xenon improves neurological outcome and reduces contusion volume following traumatic brain injury in mice. In this model, xenon application has a therapeutic time window of up to at least 3 hours. These findings support the idea that xenon may be of benefit as a neuroprotective treatment in brain trauma patients.