The bone marrow microenvironment is similarly impaired in allogeneic hematopoietic stem cell transplantation patients with early and late poor graft function
The bone marrow microenvironment is similarly impaired in allogeneic hematopoietic stem cell transplantation patients with early and late poor graft function
复制标题
异基因造血干细胞移植患者的骨髓微环境同样受到损害，早期和晚期移植物功能较差
DOI：
10.1038/bmt.2015.229
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发表时间：
2016-02-01
影响因子：
4.8
通讯作者：
Huang, X-J
中科院分区：
文献类型：
--
作者：
Kong, Y.;Wang, Y-T;Huang, X-J
移植物功能不良（PGF），包括早期和晚期PGF，是同种异体移植后的严重并发症。我们最近报道了骨髓微环境异常可能发生在晚期PGF病例中。这些异常是否发生在早期PGF中尚不清楚。为了回答这个问题，我们进行了一项巢式病例对照研究，比较了10名早期PGF患者、30名晚期PGF患者和40名无PGF患者骨髓微环境的细胞成分。采用流式细胞术、苏木精-伊红原位染色和免疫组化染色对骨髓内皮细胞、血管周围细胞和内皮细胞进行分析。与没有PGF的移植受者相比，早期和晚期PGF患者在这些细胞类型上有相似的异常。然而，上述骨髓微环境元素在早期和晚期PGF患者之间没有显著差异。我们的数据表明，类似的骨髓微环境异常可能发生在同种异体移植后PGF的早期和晚期。细胞方法，如间充质干细胞的管理，有望成为早期或晚期PGF患者的有益治疗策略。
Poor graft function (PGF), including early and late PGF, is a serious complication following allotransplant. We recently reported that bone marrow microenvironment abnormalities may occur in cases of late PGF. Whether these abnormalities occur in early PGF remains unknown. To answer this question, we performed a nested case–control study comparing cellular elements of the bone marrow microenvironment in 10 subjects with early PGF, 30 subjects with late PGF and 40 subjects without PGF. Bone marrow endosteal cells, perivascular cells and endothelial cells were analyzed by flow cytometry and by hematoxylin–eosin and immunohistochemical staining in situ. Subjects with early and late PGF had similar abnormalities in these cell types compared with transplant recipients without PGF. However, none of the aforementioned elements of the bone marrow microenvironment were significantly different between early and late PGF patients. Our data suggest that similar abnormalities in the bone marrow microenvironment may occur in early and late PGF post allotransplant. Cellular approaches, such as the administration of mesenchymal stem cells, promise to be beneficial therapeutic strategies in patients with early or late PGF.