A novel phenotypic assay of hepatitis B virus polymerase with extensive site-specific mutagenesis
A novel phenotypic assay of hepatitis B virus polymerase with extensive site-specific mutagenesis
复制标题
具有广泛位点特异性突变的乙型肝炎病毒聚合酶的新型表型测定
DOI：
10.1007/s12250-016-3932-0
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发表时间：
2017-03
影响因子：
5.5
通讯作者：
Hu Jie-Li
中科院分区：
文献类型：
--
作者：
Liu Ya;Luo Ying-Ying;Cai Xue-Fei;Long Quan-Xin;Gan Chun-Yang;Yang Liu-Qing;Guo Haitao;Huang Ai-Long;Zhang Wen-Lu;Hu Jie-Li
总之，我们开发了一种新的诱变表型检测方法，具有以下特点：（1）通过PCR步骤和FSR获得广泛的位点特异性突变;（2）产生表达不同聚合酶突变体的慢病毒库
In summary, we developed a novel mutagenesis phenotypic assay with the following characteristics: (1) acquisition of extensive site-specific mutations through a PCR step and FSR; (2) production of a lentivirus pool expressing different polymerase mutants