miR-71 and miR-263 Jointly Regulate Target Genes Chitin synthase and Chitinase to Control Locust Molting.
miR-71 and miR-263 Jointly Regulate Target Genes Chitin synthase and Chitinase to Control Locust Molting.
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miR-71和miR-263联合调控靶基因几丁质合酶和几丁质酶控制蝗虫蜕皮
DOI：
10.1371/journal.pgen.1006257
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发表时间：
2016-08
期刊：
影响因子：
4.5
通讯作者：
Kang L
中科院分区：
文献类型：
--
作者：
Yang M;Wang Y;Jiang F;Song T;Wang H;Liu Q;Zhang J;Zhang J;Kang L
几丁质合成酶和几丁质酶在昆虫蜕皮过程中几丁质的合成和降解中起着重要作用。Dicer-1的沉默导致成熟miRNA水平降低，并严重阻碍了飞蝗的蜕皮。然而，miRNAs在蝗虫蜕皮过程中的调控机制仍不清楚。本研究发现，在若虫蜕皮过程中，几丁质合成酶（chitinesynthase，CHS）和几丁质酶（chitinase，CHT）这两个几丁质代谢的关键酶受到miR-71和miR-263的调控。CHS 1的编码序列和CHT 10的3 '-非翻译区分别含有miR-71和miR-263的功能结合位点。miR-71/miR-263与其靶基因在表皮细胞中呈现细胞共定位，并分别与蝗虫体壁中的CHS 1和CHT 10直接相互作用。注射miR-71和miR-263 agomir抑制了CHS 1和CHT 10的表达，从而改变了新的和旧的角质层的几丁质产生，导致蝗虫蜕皮缺陷表型。出乎意料的是，减少miR-71和miR-263的表达增加了CHS 1和CHT 10 mRNA的表达，并导致类似于miRNA递送诱导的蜕皮缺陷。本研究揭示了两种miRNAs在几丁质生物合成和降解中的新功能和平衡调节模式，为蝗虫蜕皮过程的分子机制提供了新的见解。
Chitin synthase and chitinase play crucial roles in chitin biosynthesis and degradation during insect molting. Silencing of Dicer-1 results in reduced levels of mature miRNAs and severely blocks molting in the migratory locust. However, the regulatory mechanism of miRNAs in the molting process of locusts has remained elusive. In this study, we found that in chitin metabolism, two crucial enzymes, chitin synthase (CHS) and chitinase (CHT) were regulated by miR-71 and miR-263 during nymph molting. The coding sequence of CHS1 and the 3’-untranslated region of CHT10 contain functional binding sites for miR-71 and miR-263, respectively. miR-71/miR-263 displayed cellular co-localization with their target genes in epidermal cells and directly interacted with CHS1 and CHT10 in the locust integument, respectively. Injections of miR-71 and miR-263 agomirs suppressed the expression of CHS1 and CHT10, which consequently altered chitin production of new and old cuticles and resulted in a molting-defective phenotype in locusts. Unexpectedly, reduced expression of miR-71 and miR-263 increased CHS1 and CHT10 mRNA expression and led to molting defects similar to those induced by miRNA delivery. This study reveals a novel function and balancing modulation pattern of two miRNAs in chitin biosynthesis and degradation, and it provides insight into the underlying molecular mechanisms of the molting process in locusts.