Antigenic divergence between Bordetella pertussis clinical isolates from Moscow, Russia, and vaccine strains
Antigenic divergence between Bordetella pertussis clinical isolates from Moscow, Russia, and vaccine strains
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DOI：
10.1128/cvi.00294-06
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发表时间：
2007-03-01
影响因子：
--
通讯作者：
Mooi, Frits R.
中科院分区：
文献类型：
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作者：
Borisova, Olga;Kombarova, Svetlana Yu;Mooi, Frits R.
我们分析了1948年至2004年俄罗斯莫斯科百日咳患者分离百日咳杆菌株ptxA、prn、fim2和fim3等位基因频率的时间变化。用于全细胞疫苗的三种菌株含有等位基因ptxA2、ptxA4、prn1、fim2-1和fim3A。疫苗型等位基因ptxA （ptxA2和ptrA4）是所有预防接种菌株的特征，在20世纪60年代和70年代分离的菌株中占96%。在20世纪70年代初，ptxA2和ptxA4被ptxA1等位基因所取代。从20世纪80年代到现在，ptxA1菌株在百日咳人群中占主导地位。所有预防接种菌株都含有与疫苗型等位基因相对应的prn1等位基因。随后几年，携带prn1等位基因的菌株比例下降，携带prn3和prn2等位基因的菌株比例上升。2002 - 2004年百日咳人群以prn2或prn3型为主。在1960 - 1989年间分离的所有疫苗株和92%的菌株中均发现疫苗型等位基因fim2-1和fim3A。20世纪80年代初首次观察到fim2-2和fim3B等位基因。在随后的几年里，这些菌株占主导地位。在莫斯科地区观察到的疫苗株和临床分离株之间的抗原差异，加上免疫力下降，可能解释了百日咳的持续存在，尽管疫苗覆盖率很高。结果表明，百日咳疫苗株的选择必须基于对百日咳人群的深入研究。
We analyzed temporal changes in the frequencies of the ptxA, prn,fim2, and fim3 alleles in Bordetella pertussis strains isolated from pertussis patients in Moscow, Russia, from 1948 to 2004. The three strains used for the whole-cell vaccine harbored the alleles ptxA2,ptxA4,prn1,fim2-1, and fim3A. Vaccine-type allelles of ptxA (ptxA2 and ptrA4) were characteristic for all prevaccination strains and for 96% of the strains isolated in the 1960s and 1970s. At the beginning of the 1970s, ptxA2 and ptxA4 were replaced by the ptxA1 allele. In the 1980s and to the present, strains with ptxA1 were predominant in the B. pertussis population. All prevaccination strains harbored the prn1 allele, which corresponds to the vaccine-type allele. In subsequent years, the proportion of strains with the prn1 allele decreased and the proportion of prn3 and prn2 strains increased. From 2002 to 2004 strains with prn2 or prn3 were predominant in the B. pertussis population. The vaccine-type alleles fim2-1 and fim3A were found in all prevaccination strains and in 92% of the strains isolated from 1960 to 1989. The fim2-2 and fim3B allelles were first observed at the beginning of the 1980s. In subsequent years, these strains became predominant. Together with waning immunity, the antigenic divergence between vaccine strains and clinical isolates observed in the Moscow area may explain the persistence of pertussis, despite the high rates of vaccine coverage. The results demonstrate that the selection of B. pertussis strains for vaccine manufacturing must be based on a thorough study of the B. pertussis population.