Electrochemiluminescent Determination of Cancer Cells Based on Aptamers, Nanoparticles, and Magnetic Beads
Electrochemiluminescent Determination of Cancer Cells Based on Aptamers, Nanoparticles, and Magnetic Beads
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基于适配体、纳米粒子和磁珠的癌细胞电化学发光测定
DOI：
10.1002/chem.201104019
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发表时间：
2012-06-01
影响因子：
4.3
通讯作者：
Liu, Haitao
中科院分区：
文献类型：
--
作者：
Ding, Caifeng;Wei, Shuang;Liu, Haitao
在本文中，我们报告了一种无聚合酶链反应（PCR）的电化学发光（ECL）方法，该方法使用ECL纳米探针以高灵敏度测定癌细胞。ECL纳米探针由金纳米颗粒（AuNPs）、接头DNA和三（2，2 '-联吡啶）钌（TBR）标记的信号DNA组成。通过Au？S债券。连接体DNA可以与装载在磁珠（MB 1）上的癌细胞的适体部分杂交以构建磁性生物复合物。在癌细胞的存在下，适体以更高的亲和力与癌细胞缀合。ECL纳米探针从生物复合物中释放，随后与加载到另一个磁珠（MB 2）上的捕获DNA杂交以形成磁性纳米复合物。由于其优异的磁性能，纳米复合材料可以容易地分离并牢固地附着在电极上。负载在纳米复合材料上的TBR的ECL强度直接反映了癌细胞的量。以伯基特淋巴瘤细胞系（拉莫斯细胞）为模型，在5 ~ 100个细胞范围内，ECL响应与细胞浓度成正比。ml-1;检测限低至5个细胞？ml-1的拉莫斯细胞。本文所述的方法是理想的癌症诊断，由于其高灵敏度，简单，低成本。
Herein we report a polymerase chain reaction (PCR)-free electrochemiluminescence (ECL) approach that uses ECL nanoprobes for the determination of cancer cells with high sensitivity. The ECL nanoprobe consists of gold nanoparticles (AuNPs), linker DNA, and tris(2,2'-bipyridyl)ruthenium (TBR)-labeled signal DNA. The linker DNA and signal DNA were modified on the surface of the AuNPs through Au?S bonds. The linker DNA can partly hybridize with the aptamers of cancer cells loaded onto the magnetic beads (MB1) to construct the magnetic biocomplexes. In the presence of the cancer cells, the aptamers conjugated with the cancer cells with higher affinity. The ECL nanoprobe was released from the biocomplexes and subsequently hybridized with the capture DNA loaded onto another magnetic bead (MB2) to form the magnetic nanocomposite. The nanocomposites can be easily separated and firmly attached to an electrode on account of their excellent magnetic properties. The ECL intensity of the TBR loaded onto the nanocomposites directly reflected the amount of cancer cells. By using cell lines of Burkitts lymphoma (Ramos cells) as a model, the ECL response was proportional to the cell concentration in the range from 5 to 100 cells?ml-1; a limit of detection as low as 5 cells?ml-1 of Ramos cells could be achieved. The proposed method described here is ideal for the diagnosis of cancers due to its high sensitivity, simplicity, and low cost.