CHARACTERIZATION OF A 40S RIBOSOMAL-SUBUNIT COMPLEX IN POLYRIBOSOMES OF SACCHAROMYCES-CEREVISIAE TREATED WITH CYCLOHEXIMIDE
CHARACTERIZATION OF A 40S RIBOSOMAL-SUBUNIT COMPLEX IN POLYRIBOSOMES OF SACCHAROMYCES-CEREVISIAE TREATED WITH CYCLOHEXIMIDE
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DOI：
10.1128/mcb.1.1.51
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发表时间：
1981-01-01
影响因子：
5.3
通讯作者：
DAHLBERG, AE
中科院分区：
文献类型：
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作者：
HELSER, TL;BAAN, RA;DAHLBERG, AE
在特定条件下，放线菌酮处理S。酿酒酵母引起一种称为半聚体的多核糖体的积累。半聚体的有限核糖核酸酶消化从鉴定为40S核糖体亚基的多核糖体释放颗粒。半聚体是多聚核糖体，其含有与mRNA连接的额外的40S核糖体亚基。半聚体的蛋白质凝胶电泳分析显示许多非核糖体蛋白。其中两种蛋白质与酵母起始因子eIF2的亚基共迁移。
Under specific conditions cycloheximide treatment of S. cerevisiae caused the accumulation of a type of polyribosome called halfmer. Limited ribonuclease digestion of halfmers released particles from the polyribosomes identified as 40S ribosomal subunits. Halfmers are polyribosomes containing an additional 40S ribosomal subunit attached to the mRNA. Protein gel electrophoretic analysis of halfmers revealed numerous nonribosomal proteins. Two of these proteins comigrate with subunits of yeast initiation factor eIF2.