Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein
Differential expression of two paralogous genes of Bacillus subtilis encoding single-stranded DNA binding protein
复制标题
DOI：
10.1128/jb.186.4.1097-1105.2004
复制
发表时间：
2004-02-01
影响因子：
3.2
通讯作者：
Kuipers, OP
中科院分区：
文献类型：
--
作者：
Lindner, C;Nijland, R;Kuipers, OP
枯草芽孢杆菌基因组包含两个旁系同源的单链DNA结合蛋白（SSB）基因，ssb和ywpH，其显示不同的表达模式。主要的ssb基因在指数生长过程中强烈表达，并与编码核糖体蛋白S6和S18的基因共调控。在B中观察到的基因组织rpsF-ss B-rpsR。枯草杆菌存在于许多革兰氏阳性菌和一些革兰氏阴性菌中，但不在大肠杆菌中。ssb基因对于细胞活力是必需的，并且像其他SSB一样，其表达在SOS响应期间升高。相比之下，旁系同源的ywpH基因转录从自己的启动子在开始的稳定期仅在基本培养基中。其表达是ComK依赖性的，并且其基因产物是最佳自然转化所需的。
The Bacillus subtilis genome comprises two paralogous single-stranded DNA binding protein (SSB) genes, ssb and ywpH, which show distinct expression patterns. The main ssb gene is strongly expressed during exponential growth and is coregulated with genes encoding the ribosomal proteins S6 and S18. The gene organization rpsF-ssb-rpsR as observed in B. subtilis is found in many gram-positive as well as some gram-negative bacteria, but not in Escherichia coli. The ssb gene is essential for cell viability, and like other SSBs its expression is elevated during SOS response. In contrast, the paralogous ywpH gene is transcribed from its own promoter at the onset of stationary phase in minimal medium only. Its expression is ComK dependent and its gene product is required for optimal natural transformation.