Screening differential circular RNA expression profiles reveal that hsa_circ_0128298 is a biomarker in the diagnosis and prognosis of hepatocellular carcinoma.
Screening differential circular RNA expression profiles reveal that hsa_circ_0128298 is a biomarker in the diagnosis and prognosis of hepatocellular carcinoma.
复制标题
筛选差异环状RNA表达谱揭示hsa_circ_0128298是肝细胞癌诊断和预后的生物标志物。
DOI：
10.2147/cmar.s166740
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发表时间：
2018
影响因子：
3.3
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Chen D;Zhang C;Lin J;Song X;Wang H
本研究旨在分析环状RNA（circRNA）hsa_circ_0128298在肝细胞癌（HCC）诊断和预后中的价值。使用来自HCC患者的三对癌组织和非癌组织，使用circRNA微阵列测量总体circRNA表达。芯片分析显示，两个circRNA在三对癌组织和非癌组织中差异表达。通过实时聚合酶链反应进一步证实了两种代表性circRNA，如hsa_circ_0128298和hsa_circ_0091582的较高水平。此外，进一步分析hsa_circ_0128298的表达水平与HCC患者临床病理特征之间的关系。通过受试者工作特征曲线（ROC）分析，确定其临床诊断价值。使用考克斯回归模型确定患者预后的独立预后因素。生存数据采用Kaplan-Meier法进行分析，差异性采用log-rank检验。双侧P值<0.05被认为具有统计学显著性。hsa_circ_0128298在肝癌组织中的表达水平显著高于癌旁组织（P<0.001）。此外，hsa_circ_0128298是一个诊断因素，ROC曲线下面积为0.668（95%CI =0.503-0.794，P<0.001）。敏感性和特异性分别为0.716和0.815。AFP和hsa_circ_0128298表达水平是独立的预后因素。hsa_circ_0128298低表达患者的总生存率显著高于hsa_circ_0128298高表达患者。hsa_circ_0128298可能促进增殖和转移，并可能代表HCC患者的新诊断和预后生物标志物。然而，需要更大样本量的研究来证实我们的结论。
The aim of this study was to analyze the diagnostic and prognostic values of the circular RNA (circRNA) hsa_circ_0128298 in hepatocellular carcinoma (HCC). The global circRNA expression was measured using circRNA microarray using three pairs of cancer and noncancerous tissues from HCC patients. The microarray analysis revealed that two circRNAs were differentially expressed in the three pairs of cancerous and noncancerous tissues. The higher levels of two representative circRNAs, such as hsa_circ_0128298 and hsa_circ_0091582, were further confirmed by real-time polymerase chain reaction. In addition, the association between the expression level of hsa_circ_0128298 and the clinicopathological features of patients with HCC was further analyzed. The clinical diagnosis value was confirmed by receiver operating characteristic (ROC) curve analysis. Independent prognostic factors of patient outcome were identified using the Cox regression model. The survival data were analyzed by the Kaplan–Meier method, and the differences were evaluated using log-rank tests. Two-sided P-values <0.05 were considered statistically significant. The expression levels of hsa_circ_0128298 in HCC were significantly higher than those of paratumorous tissues (P<0.001). Additionally, hsa_circ_0128298 was a diagnostic factor, with the area under the ROC curve of 0.668 (95% CI =0.503–0.794, P<0.001). The sensitivity and specificity values were 0.716 and 0.815, respectively. The AFP and hsa_circ_0128298 expression levels were independent prognostic factors. The overall survival of patients with low hsa_circ_0128298 expression was significantly higher than that of patients with high hsa_circ_0128298 expression. hsa_circ_0128298 may promote proliferation and metastasis and potentially represents a novel diagnostic and prognostic biomarker for HCC patients. However, studies with larger sample size are needed to confirm our conclusion.