Genetic Linkage Map of the Eastern Oyster Crassostrea virginica Gmelin
Genetic Linkage Map of the Eastern Oyster Crassostrea virginica Gmelin
复制标题
DOI：
10.2307/1543603
复制
发表时间：
2003-06
期刊：
影响因子：
--
通讯作者：
Ziniu Yu;Ximing Guo
中科院分区：
文献类型：
--
作者：
Ziniu Yu;Ximing Guo
扩增片段长度多态性 (AFLP) 以及一些微卫星和 I 型标记被用于东部牡蛎 Crassostrea virginica Gmelin 的连锁分析。选择 17 个 AFLP 引物组合与两个亲本及其 81 个后代进行连锁分析。 17个引物组合产生396个多态性标记，其中282个在两个亲本中分离。卡方分析表明，259 个 (91.8%) 标记以孟德尔比例分离，而其他 23 个 (8.2%) 显示出显着 (P < 0.05) 的分离扭曲，主要是由于纯合子缺陷，可能是由于有害的隐性基因。分别使用来自雄性和雌性亲本的 158 个和 133 个分离标记（包括一些微卫星和 I 型标记）构建了中等密集的连锁图谱。雄性框架图由12个连锁群的114个标记组成，覆盖647 cM。雌性图谱有12个连锁群中的84个标记，长度为904 cM。男性图谱的估计基因组长度为 858 cM，女性图谱的估计基因组长度为 1296 cM。当考虑所有连锁标记时，观察到的男性和女性图谱的基因组覆盖率为 84%。本研究中观察到的遗传图谱比细胞遗传学图谱长，可能是因为标记密度低。
Amplified fragment length polymorphisms (AFLPs), along with some microsatellite and Type I markers, were used for linkage analysis in Crassostrea virginica Gmelin, the eastern oyster. Seventeen AFLP primer combinations were selected for linkage analysis with two parents and their 81 progeny. The 17 primer combinations produced 396 polymorphic markers, and 282 of them were segregating in the two parents. Chi-square analysis indicated that 259 (91.8%) markers segregated in Mendelian ratio, while the other 23 (8.2%) showed significant (P < 0.05) segregation distortion, primarily for homozygote deficiency and probably due to deleterious recessive genes. Moderately dense linkage maps were constructed using 158 and 133 segregating markers (including a few microsatellite and Type I markers) from male and female parents, respectively. The male framework map consisted of 114 markers in 12 linkage groups, covering 647 cM. The female map had 84 markers in 12 linkage groups with a length of 904 cM. The estimated genome length was 858 cM for the male map and 1296 cM for the female map. The observed genome coverage was 84% for the male and female map when all linked markers were considered. Genetic maps observed in this study are longer than the cytogenetic map, possibly because of low marker density.