Active CMOS array sensor for time-resolved fluorescence detection
Active CMOS array sensor for time-resolved fluorescence detection
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DOI：
10.1109/jssc.2006.883316
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发表时间：
2006-11-01
影响因子：
5.4
通讯作者：
Levicky, Rastislav
中科院分区：
文献类型：
--
作者：
Patounakis, George;Shepard, Kenneth L.;Levicky, Rastislav
基于荧光检测的表面传感检测在环境和生物医学诊断中已变得司空见惯。传统的阵列扫描仪是昂贵、大型且复杂的仪器。本文描述了用于基于荧光的检测的有源 CMOS 生物传感器基板的设计，该基板无需外部读取器即可实现时间选通、时间分辨荧光光谱。该阵列对低至 1.15 x 10(8)/cm(2) 的光子密度敏感，动态范围超过 74 dB，并具有亚纳秒定时分辨率。灵敏度是通过二次采样和平均来实现的。
Surface-based sensing assays based on fluorescence-based detection have become commonplace for both environmental and biomedical diagnostics. Traditional array scanners are expensive, large, and complex instruments. This paper describes the design of an active CMOS biosensor substrate for fluorescence-based assays that enables time-gated, time-resolved fluorescence spectroscopy without the need for an external reader. The array is sensitive to photon densities as low as 1.15 x 10(8)/cm(2), has a dynamic range of over 74 dB, and has subnanosecond timing resolution. Sensitivity is achieved through subsampling and averaging.