Enhanced hematopoietic activity accompanies parasite expansion in the spleen and bone marrow of mice infected with Leishmania donovani
Enhanced hematopoietic activity accompanies parasite expansion in the spleen and bone marrow of mice infected with Leishmania donovani
复制标题
DOI：
10.1128/iai.68.4.1840-1848.2000
复制
发表时间：
2000-04-01
影响因子：
3.1
通讯作者：
Kaye, PM
中科院分区：
文献类型：
--
作者：
Coterell, SEJ;Engwerda, CR;Kaye, PM
在本研究中，我们分析了感染杜氏利什曼原虫的BALB/c和SCID小鼠的脾、骨髓和血液中的造血活性。我们的分析表明，感染诱导祖细胞快速但短暂地动员进入循环，与粒细胞/巨噬细胞集落刺激因子(GM-CSF)和MIP-1α水平升高有关。从感染后14天到28天，当寄生虫在脾和骨髓开始扩张时，造血祖细胞的频率和细胞周期活性在这些器官中都显著增加，尤其是CFU-粒细胞和单核细胞。这与GM-CSF、M-CSF和G-CSF的mRNA积累增加有关，但与IL-3无关。我们的数据还表明，通过祖细胞群体频率及其细胞周期活动水平的变化来评估造血活性，可以在T细胞非依赖性和T细胞依赖性以及器官特异性的方式下进行调节。总而言之，这些数据增加了我们对多诺瓦尼乳杆菌能够持续存在的器官中发生的长期变化的了解。
In this study, we have analyzed hematopoietic activity in the spleen, bone marrow, and blood of BALB/c and scid mice infected with Leishmania donovani. Our analysis demonstrates that infection induces a rapid but transient mobilization of progenitor cells into the circulation, associated with elevated levels of granulocyte/ macrophage colony-stimulating factor (GM-CSF) and MIP-1 alpha. From 14 to 28 days postinfection, when parasite expansion begins in the spleen and bone marrow; both the frequency and cell cycle activity of hematopoietic progenitors, particulary CFU-granulocyte, monocyte, are dramatically increased in these organs. This is associated with increased accumulation of mRNA far GM-CSF, M-CSF, and G-CSF, but not interleukin-3. Our data also illustrate that hematopoietic activity, as assessed by changes in the frequency of progenitor cell populations and their levels of cell cycle activity, can be regulated in both a T-cell-independent and T-cell-dependent, as well as in an organ-specific, manner. Collectively, these data add to our knowledge of the long-term changes which occur in organs in which L. donovani is able to persist.