Interleukin-1alpha stimulates steroidogenic acute regulatory protein expression via p38 MAP kinase in immature rat Leydig cells.
Interleukin-1alpha stimulates steroidogenic acute regulatory protein expression via p38 MAP kinase in immature rat Leydig cells.
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DOI：
10.1677/jme.0.0300059
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发表时间：
2003-02
影响因子：
3.5
通讯作者：
K. Svechnikov;DM Stocco;O. Soder
中科院分区：
文献类型：
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作者：
K. Svechnikov;DM Stocco;O. Soder
我们在体外研究了在未成熟（40日龄）和成年间质细胞中，类固醇生成急性调节（StAR）蛋白参与白细胞介素-1 α (il -1 α)诱导的类固醇生成。此外，我们还研究了il -1 α介导的信号通路在未成熟间质细胞中调控StAR的表达。il -1 α在未成熟而非成年间质细胞中以剂量和时间依赖的方式刺激雄激素产生和StAR蛋白表达。通过p38 MAP激酶特异性抑制剂SB203580和PD169316预处理细胞可以阻止il -1 α的这些作用，这表明该激酶是未成熟间质细胞中il -1 α信号传导的重要组成部分。目前的研究结果表明，il -1 α是大鼠出生后25天睾丸组成性产生的一种重要的旁分泌调节因子，可调节大鼠出生后间质细胞的成熟。调节StAR蛋白的表达是il -1 α促进未成熟间质细胞向成体细胞分化的可能机制之一。
We have investigated the involvement of the steroidogenic acute regulatory (StAR) protein in interleukin-1alpha (IL-1alpha)-induced steroidogenesis in immature (40-day-old) and adult Leydig cells in vitro. Further, IL-1alpha-mediated signaling pathway(s) controlling StAR expression in immature Leydig cells were also studied. IL-1alpha stimulated both androgen production and StAR protein expression in a dose- and time-dependent manner in immature but not adult Leydig cells. These effects of IL-1alpha were prevented by pretreatment of the cells with the specific inhibitors of the p38 MAP kinase, SB203580 and PD169316, suggesting that this kinase is an important part of IL-1alpha signaling in the immature Leydig cell. The present results suggest that IL-1alpha, which is constitutively produced by the rat testis from postnatal day 25, is an important paracrine regulator of postnatal Leydig cell maturation. Regulation of StAR protein expression is one of the possible mechanisms by which IL-1alpha contributes to the differentiation of immature Leydig cells into adult cells.