Evaluation of activity and inhibition effects on Anammox process by batch tests based on the nitrogen gas production
Evaluation of activity and inhibition effects on Anammox process by batch tests based on the nitrogen gas production
复制标题
DOI：
10.1016/j.enzmictec.2006.06.018
复制
发表时间：
2007-03-05
影响因子：
3.4
通讯作者：
Jetten, M. S. M.
中科院分区：
文献类型：
--
作者：
Dapena-Mora, A.;Fernandez, I.;Jetten, M. S. M.
为了评价厌氧氨氧化（Anaminox）工艺在工业规模上的适用性，有必要研究工业废水中常见的不同化合物对最大特定厌氧氨氧化活性（SAA）的毒性影响。本研究的重点是应用批量试验来确定不同条件下的最大SAA。批量试验是基于对氮气产量的测量。测定SAA最大值的初始条件为：30℃，pH 7.8，振动速度150 rpm，生物量浓度1 g VSS L-1。用含氮化合物的质量天平对方法的准确度进行了评价，得到的误差小于7%。试验的初始生物量浓度（0.5-2.0 g VSS L-1）和S-0/X-0比值（0.018 ~ 0.140 g NO2—N g VSS-1）对估算的SAA都没有显著影响。添加第二次进料可使其增加20 +/- 10%左右。随后将该方法应用于不同化合物（NH4+、NO2-、NO3-、NaCl、SO42-、S2-、絮凝剂等）对厌氧氨氧化过程的抑制作用的研究。研究了氯霉素（反硝化抑制剂）和烯基硫脲（硝化抑制剂）对厌氧氨氧化菌活性的影响，以区分厌氧氨氧化菌的硝化和反硝化活性。所开发的批量实验不仅可以确定某些污泥的最大SAA，还可以确定某些被试化合物的抑制效果。(c) 2006爱思唯尔公司版权所有。
In order to assess the applicability at industrial-scale of the anaerobic ammonium oxidation (Anaminox) process it is necessary to study the toxic effects on the maximum specific Anammox activity (SAA) of different compounds commonly present in industrial effluents. The present study was focused on the application of batch tests to determine the maximum SAA in different conditions. The batch tests were based on the measurement of nitrogen gas production. The initial conditions for the tests established to obtain the maximum value of the measured SAA were: 30 degrees C, pH 7.8, shaking speed 150 rpm, and biomass concentration 1 g VSS L-1. The accuracy of the method was evaluated by mass balances of the nitrogen compounds and the obtained errors were smaller than 7%. Neither the initial biomass concentrations tested (0.5-2.0 g VSS L-1) nor the S-0/X-0 ratio between 0.018 and 0.140 g NO2--N g VSS-1 had significant influence on the estimated SAA. The addition of a second feeding tended to increase it around 20 +/- 10%. The developed method is afterwards applied to the study of the inhibition caused on the Anammox process by different compounds (NH4+, NO2-, NO3-, NaCl, SO42-, S2-, flocculant, etc.). The effects of chloramphenicol (inhibitor of the denitrifying process) and allylthiourea (inhibitor of the nitrifying process) were tested in order to be used to distinguish between the Anammox activity from nitrifying and denitrifying activities. The developed batch experiments were found suitable to establish not only the maximum SAA of certain sludge but also the inhibitory effects of certain tested compounds. (c) 2006 Elsevier Inc. All rights reserved.