Cooling of a vial in a snapfreezing device without using sacrificial cryogens
Cooling of a vial in a snapfreezing device without using sacrificial cryogens
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在速冻装置中冷却小瓶，无需使用牺牲制冷剂
DOI：
10.1038/s41598-019-40115-6
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发表时间：
2019
影响因子：
4.6
通讯作者：
S. Vanapalli
中科院分区：
文献类型：
--
作者：
M. A. J. van Limbeek;S. Jagga;H. Holland;K. Ledeboer;M. ter Brake;S. Vanapalli
在分子医学中，需要新鲜和冷冻的高质量患者生物样本，以鉴定分子水平上与疾病相关的机制。一种常见的冷却程序是将含有液氮等冷却液中的小瓶中的组织浸入。此过程不友好，并且辛苦了，因为从切除时间到冻结的滞后时间取决于医院内的后勤组织结构。此外，必须在组织切除后尽快进行Snapfreezing，以保留分子测试的组织质量。在此，我们报告了一种电力的快速冻结装置，作为淬灭液体氮的小瓶的替代方法，因此可以直接在获取组织的位置使用。该设备还促进了冻结条件对样品中各种分子过程的影响的研究。快照冻结装置中的小瓶的冷却实验表明，与液氮中的淬火相似或更快的冷却速率是可行的。我们进行了一些设定点条件的实验，并将结果与数学模型进行了比较。
A fresh and frozen high-quality patient bio-sample is required in molecular medicine for the identification of disease-associated mechanism at molecular levels. A common cooling procedure is immersing the tissue enclosed in a vial in a coolant such as liquid nitrogen. This procedure is not user friendly and is laborious as reducing the lag time from excision time to freezing depends on the logistic organizational structure within a hospital. Moreover snapfreezing must be done as soon as possible after tissue excision to preserve the tissue quality for molecular tests. Herein, we report an electrically powered snap freezing device as an alternative to quenching the vial in liquid nitrogen and therefore can be used directly at the location where the tissue is acquired. This device also facilitates the study of the effect of freezing conditions on the various molecular processes in the samples. Cooling experiments of a vial in the snap freezing device show that the cooling rates similar to or faster than quenching in liquid nitrogen are feasible. We performed experiments with several set point conditions and compared the results with a mathematical model.
影响因子：
12.8
作者：
Labots, Mariette;Gotink, Kristy J.;Dekker, Henk;Azijli, Kaamar;van der Mijn, Johannes C.;Huijts, Charlotte M.;Piersma, Sander R.;Jimenez, Connie R.;Verheul, Henk M. W.
通讯作者：
Verheul, Henk M. W.