RELEASE OF ACTIVE CYTOKININ BY A BETA-GLUCOSIDASE LOCALIZED TO THE MAIZE ROOT-MERISTEM
RELEASE OF ACTIVE CYTOKININ BY A BETA-GLUCOSIDASE LOCALIZED TO THE MAIZE ROOT-MERISTEM
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DOI：
10.1126/science.8235622
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发表时间：
1993-11-12
期刊：
影响因子：
56.9
通讯作者：
PALME, K
中科院分区：
文献类型：
--
作者：
BRZOBOHATY, B;MOORE, I;PALME, K
由克隆的玉米互补DNA(Zm-p60.1)编码的β-葡萄糖苷将生物活性激素结合的细胞分裂素-O-葡萄糖苷和激动素-N3-葡萄糖苷切割，释放出活性细胞分裂素。瞬时表达Zm-p60.1的烟草原生质体可以利用失活的细胞分裂素葡萄糖苷启动细胞分裂。在表达载体可能消失后，原生质体对细胞分裂素糖苷的反应维持生长的能力无限期地存在。在玉米幼苗的根中，Zm-p60.1定位于分生组织细胞，并可能在体内发挥作用，为发育中的玉米胚胎提供活性细胞分裂素。
A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60.1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3-glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin glucosides to initiate cell division. The ability of protoplasts to sustain growth in response to cytokinin glucosides persisted indefinitely after the likely disappearance of the expression vector. In the roots of maize seedlings, Zm-p60.1 was localized to the meristematic cells and may function in vivo to supply the developing maize embryo with active cytokinin.