Neutrophil extracellular Taps play an important role in clearance of Streptococcus suis in vivo
Neutrophil extracellular Taps play an important role in clearance of Streptococcus suis in vivo
复制标题
中性粒细胞胞外 Taps 在猪链球菌体内清除中发挥重要作用
DOI：
10.1111/1348-0421.12367
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发表时间：
2016-04-01
影响因子：
2.6
通讯作者：
Zhang, Anding
中科院分区：
文献类型：
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作者：
Zhao, Jianqing;Lin, Lan;Zhang, Anding
猪链球菌感染可在体外诱导中性粒细胞胞外陷阱(NETS)的形成，但NETS介导的杀伤作用在猪链球菌体内致病机制中的作用尚不清楚。本研究的结果表明，在猪链球菌感染的小鼠模型中可以诱导细胞外DNA纤维。一种破坏其结构的核酸酶被用来评估Net在猪链球菌感染中的作用。核酸酶处理后，猪链球菌感染后细菌载量增加，血清肿瘤坏死因子浓度升高，说明Nets结构在猪链球菌清除和炎症过程中起重要作用。此外，核酸酶治疗可导致更严重的临床症状和更高的猪链球菌感染死亡率。这些发现表明，Nets结构有助于抵御猪链球菌感染。
Streptococcus suis infection induces formation of neutrophil extracellular traps (NETs) in vitro; however, the contribution of NETs-mediated killing to the pathogenesis of S. suis in vivo is yet to be elicited. The findings of the present study indicated that extracellular DNA fiber can be induced in a murine model in response to S. suis infection. A nuclease that destroys their structure was used to evaluate the role of NETs on S. suis infection. Treatment with nuclease resulted in a greater bacteria load and higher serum TNF- concentrations in response to S. suis infection, indicating that NETs structure played an essential role in S. suis clearance and inflammation. Furthermore, nuclease treatment resulted in more severe clinical signs during and higher mortality from S. suis infection. These findings indicated that NETs structure contributes to protection against S. suis infection.