Electroencephalographic coherence and cortical acetylcholine during ketamine-induced unconsciousness
Electroencephalographic coherence and cortical acetylcholine during ketamine-induced unconsciousness
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DOI：
10.1093/bja/aev095
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发表时间：
2015-06-01
影响因子：
9.8
通讯作者：
Mashour, G. A.
中科院分区：
文献类型：
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作者：
Pal, D.;Hambrecht-Wiedbusch, V. S.;Mashour, G. A.
背景：对氯胺酮麻醉期间皮质神经化学和皮质连接的了解有限。我们进行了一项系统研究，探讨氯胺酮对皮质乙酰胆碱（ACh）和脑电图一致性的影响。方法：给雄性 Sprague-Dawley 大鼠（n=11）植入电极，记录额叶、顶叶和枕叶皮质的脑电图（EEG），并使用微透析引导插管同时测量术前、术中和术后前额皮质的 ACh 浓度。氯胺酮麻醉。根据脑电图和乙酰胆碱浓度计算出的相干性和功率谱密度在有意识和无意识状态之间进行比较。翻正反射的丧失被用作无意识的替代。结果：氯胺酮引起的无意识与较高伽马带宽（> 65 Hz）中的整体功率降低（P = 0.02）、相干性的整体降低（P
Background: There is limited understanding of cortical neurochemistry and cortical connectivity during ketamine anaesthesia. We conducted a systematic study to investigate the effects of ketamine on cortical acetylcholine (ACh) and electroencephalographic coherence.Methods: Male Sprague-Dawley rats (n=11) were implanted with electrodes to record electroencephalogram (EEG) from frontal, parietal, and occipital cortices, and with a micro dialysis guide cannula for simultaneous measurement of ACh concentrations in prefrontal cortex before, during, and after ketamine anaesthesia. Coherence and power spectral density computed from the EEG, and ACh concentrations, were compared between conscious and unconscious states. Loss of righting reflex was used as a surrogate for unconsciousness.Results: Ketamine-induced unconsciousness was associated with a global reduction of power (P=0.02) in higher gamma bandwidths (>65 Hz), a global reduction of coherence (P