Similarities and differences in the innervation of mystacial vibrissal follicle-sinus complexes in the rat and cat: A confocal microscopic study
Similarities and differences in the innervation of mystacial vibrissal follicle-sinus complexes in the rat and cat: A confocal microscopic study
复制标题
DOI：
10.1002/cne.10277
复制
发表时间：
2002-07-22
影响因子：
2.5
通讯作者：
Rice, FL
中科院分区：
文献类型：
--
作者：
Ebara, S;Kumamoto, K;Rice, FL
我们的共聚焦三维分析揭示了大鼠和猫触须毛囊窦复合体（FSC）的神经支配存在实质性差异。这是首次使用抗蛋白基因产物9.5进行的研究（PGP9.5）免疫标记和共聚焦显微镜检查厚切片上的各种FSC末梢的末端分支，并比较两个物种，大鼠和猫，具有相似外观的FSC，但具有不同的探索行为，例如是否存在搅拌。在本研究中，至少有八种不同的末梢在三维上被清楚地区分：1）在网脊颈处的默克尔末梢，2）周向取向的披针形末梢，3）在环窦水平处的默克尔末梢，4）纵向取向的披针形末梢，5）棍状环状终末，6）网状末梢，7）多刺末梢，和8）囊状末梢。与此形成鲜明对比的是，大鼠环窦水平上支配默克尔细胞的每根神经纤维通常以单个相对较小的终末簇终止，而猫则以几个较大的终末簇终止。此外，个别乔木的网状末梢在大鼠分支平行的触须和分布在广泛的，重叠的领土，而那些在猫分支垂直和终止在严格限制的领土。另外，大鼠FSC的内圆锥体包含在猫中缺乏的间接的、周向取向的披针形末梢，而猫的海绵窦具有在大鼠中缺乏的间接的微粒末梢。令人惊讶的是，在这两个物种中最相似的一种神经支配类型是一组简单的，棒状的末端，位于环管的附件，以前没有被认为是形态上独特的神经支配类型。尽管大鼠和猫的FSC的基本结构相似，但神经支配的众多差异表明这些物种具有不同的触觉能力和感知能力，这可能与它们不同的触须相关探索行为有关。
Our confocal three-dimensional analyses revealed substantial differences in the innervation to vibrissal follicle-sinus complexes (FSCs) in the rat and cat. This is the first study using anti-protein gene product 9.5 (PGP9.5) immunolabeling and confocal microscopy on thick sections to examine systematically the terminal arborizations of the various FSC endings and to compare them between two species, the rat and the cat, that have similar-appearing FSCs but different exploratory behaviors, such as existence or absence of whisking. At least eight distinct endings were clearly discriminated three dimensionally in this study: 1) Merkel endings at the rete ridge collar, 2) circumferentially oriented lanceolate endings, 3) Merkel endings at the level of the ring sinus, 4) longitudinally oriented lanceolate endings, 5) club-like ringwulst endings, 6) reticular endings, 7) spiny endings, and 8) encapsulated endings. Of particular contrast, each nerve fiber that innervates Merkel cells at the level of the ring sinus in the rat usually terminates as a single, relatively small cluster of endings, whereas in the cat they terminate en passant as several large clusters of endings. Also, individual arbors of reticular endings in the rat ramify parallel to the vibrissae and distribute over wide, overlapping territories, whereas those in the cat ramify perpendicular and terminate in tightly circumscribed territories. Otherwise, the inner conical body of rat FSCs contains en passant, circumferentially oriented lanceolate endings that are lacking in the cat, whereas the cavernous sinus of the cat has en passant corpuscular endings that are lacking in the rat. Surprisingly, the one type of innervation that is the most similar in both species is a major set of simple, club-like endings, located at the attachment of the ringwulst, that had not previously been recognized as a morphologically unique type of innervation. Although the basic structure of the FSCs is similar in the rat and cat, the numerous differences in innervation suggest that these species would have different tactile capabilities and perceptions possibly related to their different vibrissa-related exploratory behaviors.