ORION: a web server for protein fold recognition and structure prediction using evolutionary hybrid profiles.
ORION: a web server for protein fold recognition and structure prediction using evolutionary hybrid profiles.
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DOI：
10.1038/srep28268
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发表时间：
2016-06-20
影响因子：
4.6
通讯作者：
Gelly JC
中科院分区：
文献类型：
--
作者：
Ghouzam Y;Postic G;Guerin PE;de Brevern AG;Gelly JC
基于比较建模的蛋白质结构预测是产生结构模型的最有效方法。ORION是一个专用的Web服务器，基于执行此任务的新策略。使用结合序列和结构进化信息的原始轮廓-轮廓方法通过ORION鉴定合适的模板。结构进化信息使用结构特征编码到图谱中，例如溶剂可及性和局部构象（使用蛋白质块），这给出了局部蛋白质结构的准确描述。ORION最近得到了改进，其结果质量提高了5%。ORION Web服务器接受单个蛋白质序列作为输入，并在几分钟内搜索同源蛋白质结构。可以挖掘诸如PDB、SCOP和HOMSTRAD的各种数据库以找到适当的结构模板。对于建模步骤，可以通过MODELLER从所选模板直接获得蛋白质3D结构，并显示全局和局部质量模型估计度量。来自CAMEO服务器的4个示例的序列和预测结构以及来自“硬”类别（T0818-D1）的最近CASP 11目标被显示为相关示例。我们的网络服务器可访问http://www.dsimb.inserm.fr/ORION/。
Protein structure prediction based on comparative modeling is the most efficient way to produce structural models when it can be performed. ORION is a dedicated webserver based on a new strategy that performs this task. The identification by ORION of suitable templates is performed using an original profile-profile approach that combines sequence and structure evolution information. Structure evolution information is encoded into profiles using structural features, such as solvent accessibility and local conformation —with Protein Blocks—, which give an accurate description of the local protein structure. ORION has recently been improved, increasing by 5% the quality of its results. The ORION web server accepts a single protein sequence as input and searches homologous protein structures within minutes. Various databases such as PDB, SCOP and HOMSTRAD can be mined to find an appropriate structural template. For the modeling step, a protein 3D structure can be directly obtained from the selected template by MODELLER and displayed with global and local quality model estimation measures. The sequence and the predicted structure of 4 examples from the CAMEO server and a recent CASP11 target from the ‘Hard’ category (T0818-D1) are shown as pertinent examples. Our web server is accessible at http://www.dsimb.inserm.fr/ORION/.