Effects of cyclosporin a and verapamil on the intracellular daunorubicin accumulation in chinese hamster ovary cells with increasing levels of drug‐resistance
Effects of cyclosporin a and verapamil on the intracellular daunorubicin accumulation in chinese hamster ovary cells with increasing levels of drug‐resistance
复制标题
环孢素a和维拉帕米对耐药水平升高的中国仓鼠卵巢细胞内柔红霉素积累的影响
DOI：
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发表时间：
1989
影响因子：
6.4
通讯作者：
K. Nooter
中科院分区：
文献类型：
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作者：
M. Silbermann;A. W. Boersma;A. L. W. Jamssen;R. Scheper;H. Herweijer;K. Nooter
Multidrug‐resistance (MDR) is characterized by the presence of a 170 kDa glycoprotein (P‐glycoprotein) in the plasma membrane. P‐glycoprotein is thought to act as an efflux pump, leading to reduced drug accumulation in MDR cells. This defect in drug accumulation can be overcome by membrane transport modulating agents (MTMAs). We determined the concentration of MTMA needed for maximal restoration of daunorubicin content in 4 Chinese hamster ovary cell lines with increasing levels of drug‐resistance using flow cytometry. Stimulation of daunorubicin accumulation occurred in a dose‐dependent manner. The required level of MTMA needed for maximal drug accumulation increased with the level of drug‐resistance. CHrA3 cells, which have a level of resistance comparable to clinical samples, needed relatively low concentrations of MTMA for maximal restoration of drug accumulation. This indicates that, in trials combining drugs and MTMAs, low dosages of MTMAs could be sufficient for optimal potentiation of cytotoxicity.
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC