Eph signaling is required for segmentation and differentiation of the somites
Eph signaling is required for segmentation and differentiation of the somites
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DOI：
10.1101/gad.12.19.3096
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发表时间：
1998-10-01
影响因子：
10.5
通讯作者：
Holder, N
中科院分区：
文献类型：
--
作者：
Durbin, L;Brennan, C;Holder, N
体细胞发生涉及到轴旁中胚层沿前后轴沿着分割成单元。在这里，我们展示了Eph和ephrin信号在体前中胚层和体节形成的模式中的作用。肝配蛋白-A-L1和肝配蛋白-B2在发育中的体节和前体节中胚层中以迭代方式表达，Eph受体EphA 4也是如此。我们已经研究了这些蛋白质的作用，通过注射RNA，编码显性负性形式的Eph受体和肝配蛋白。Eph信号传导的中断导致异常体节边界形成和肌节中myoD表达的减少或干扰。Eph家族信号传导的中断延迟了her 1和Delta D在前体中胚层中表达的正常下调，并破坏了肌源性分化。我们认为，Eph信号在翻译的图案的preomitic中胚层成体节的关键作用。
Somitogenesis involves the segmentation of the paraxial mesoderm into units along the anteroposterior axis. Here we show a role for Eph and ephrin signaling in the patterning of presomitic mesoderm and formation of the somites. Ephrin-A-L1 and ephrin-B2 are expressed in an iterative manner in the developing somites and presomitic mesoderm, as is the Eph receptor EphA4. We have examined the role of these proteins by injection of RNA, encoding dominant negative forms of Eph receptors and ephrins. Interruption of Eph signaling leads to abnormal somite boundary formation and reduced or disturbed myoD expression in the myotome. Disruption of Eph family signaling delays the normal down-regulation of her1 and Delta D expression in the anterior presomitic mesoderm and disrupts myogenic differentiation. We suggest that Eph signaling has a key role in the translation of the patterning of presomitic mesoderm into somites.