Development of novel assays for lignin degradation: comparative analysis of bacterial and fungal lignin degraders
Development of novel assays for lignin degradation: comparative analysis of bacterial and fungal lignin degraders
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DOI：
10.1039/b908966g
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发表时间：
2010-05-01
影响因子：
--
通讯作者：
Bugg, Timothy D. H.
中科院分区：
文献类型：
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作者：
Ahmad, Mark;Taylor, Charles R.;Bugg, Timothy D. H.
已经开发了两种分光光度测定法来监测植物木质纤维素的木质素成分的分解：涉及荧光改性木质素的连续荧光测定法和涉及化学硝化木质素的紫外-可见测定法。这些测定已用于分析细菌和真菌木质素降解剂中的木质素降解活性，并鉴定显示出木质素降解活性的其他土壤细菌。两种已知可充当芳香降解剂的土壤细菌：恶臭假单胞菌和红球菌。 RHA1 在这些测定中始终表现出活性，并且这些菌株在小规模实验中显示出可分解木质纤维素，产生许多单环酚类产物。使用由麦秆、松树和芒草制备的磨碎木质素，发现一些细菌木质素降解剂对木质素类型表现出特异性。这些测定可用于鉴定用于分解植物木质纤维素的新型木质素降解剂。
Two spectrophotometric assays have been developed to monitor breakdown of the lignin component of plant lignocellulose: a continuous fluorescent assay involving fluorescently modified lignin, and a UV-vis assay involving chemically nitrated lignin. These assays have been used to analyse lignin degradation activity in bacterial and fungal lignin degraders, and to identify additional soil bacteria that show activity for lignin degradation. Two soil bacteria known to act as aromatic degraders, Pseudomonas putida and Rhodococcus sp. RHA1, consistently showed activity in these assays, and these strains were shown in a small scale experiment to breakdown lignocellulose, producing a number of monocyclic phenolic products. Using milled wood lignin prepared from wheat straw, pine, and miscanthus, some bacterial lignin degraders were found to show specificity for lignin type. These assays could be used to identify novel lignin degraders for breakdown of plant lignocellulose.