Sensitive detection of trimethylamine based on dopant-assisted positive photoionization ion mobility spectrometry
Sensitive detection of trimethylamine based on dopant-assisted positive photoionization ion mobility spectrometry
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基于掺杂剂辅助正光电离离子迁移谱的三甲胺灵敏检测
DOI：
10.1016/j.talanta.2016.10.056
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发表时间：
2017
期刊：
影响因子：
6.1
通讯作者：
Tan Mingqian
中科院分区：
文献类型：
--
作者：
Cheng Shasha;Li Haitao;Jiang D;an;Chen Chuang;Zhang Tan;Li Yong;Wang Haitao;Zhou Qinghua;Li Haiyang;Tan Mingqian
生物胺是在食物腐败过程中通过酶和微生物过程产生的降解产物，其水平升高可能对消费者的健康构成危害。由于三甲胺（TMA）的挥发性和鱼腥味，是生物胺检测的良好目标。在这项研究中，我们开发了一个独立的掺杂剂辅助正光电离离子迁移谱（DAPP-IMS）快速和灵敏的检测TMA。通过添加掺杂剂增强了TMA的响应，并形成了迁移率降低为2.26 cm 2 V-1 s-1的特征产物离子。2-为了使试剂离子峰和TMA产物离子峰更好地分离，提高灵敏度，选择丁酮作为掺杂剂，对TMA标准样品的检出限为1 ppb。通过检测牡蛎和对虾在4 °C贮藏过程中产生的TMA，评价了DAAP-IMS的潜在应用价值。两种海产品的分析结果均显示出与TMA标准样品相同的特征峰，且随着储存时间的延长，TMA的强度逐渐增强。本研究的结果证明了DAPP-IMS用于定性和定量测定真实的食品样品中TMA的潜力。
Biogenic amines are degradation products generated through enzymatic and microbial processes during food spoilage, which may pose a health hazard to consumers at elevated levels. Trimethylamine (TMA) is a good target for the detection of biogenic amines due to its volatility and fishy odor. In this study, we developed a stand-alone dopant-assisted positive photoionization ion mobility spectrometry (DAPP-IMS) for rapid and sensitive detection of TMA. Response of TMA was enhanced by the addition of dopants and characteristic product ions with reduced mobility 2.26 cm2V−1s−1were formed. 2-Butaone was chosen as the dopant for better separation between reagent ion peak and TMA product ion peak as well as higher sensitivity and the limit of detections (LODs) for TMA standard sample was 1 ppb. The potential application of DAAP-IMS was evaluated by the detection of TMA generated by oyster and shrimp during 4 °C storage. Analysis of two kinds of seafood showed the same characteristic peak to TMA standard sample, and the intensity of TMA increased over the storage time. The results of this study testify to the potential of DAPP-IMS for qualitative and quantitative determination of TMA in real food samples.