Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species.
Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species.
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DOI：
10.1007/978-1-4939-2848-4_10
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Loring, Jeanne F
中科院分区：
文献类型：
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作者：
Ben-Nun, Inbar Friedrich;Montague, Susanne C;Loring, Jeanne F
对于一些高度濒危的物种来说，有繁殖能力的动物太少，无法保持足够的遗传多样性，因此需要采取非常措施来防止它们的灭绝。细胞重编程是捕获单个动物基因组作为诱导多能干细胞（iPSCs）的一种手段，这可能最终促进遗传物质重新引入育种群体。在这里，我们描述了一种从哺乳动物濒危物种的成纤维细胞中生成iPSCs的方法。
For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent their extinction. Cellular reprogramming is a means to capture the genomes of individual animals as induced pluripotent stem cells (iPSCs), which may eventually facilitate reintroduction of genetic material into breeding populations. Here, we describe a method for generating iPSCs from fibroblasts of mammalian endangered species.