OsPP2C09, a negative regulatory factor in abscisic acid signalling, plays an essential role in balancing plant growth and drought tolerance in rice
OsPP2C09, a negative regulatory factor in abscisic acid signalling, plays an essential role in balancing plant growth and drought tolerance in rice
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OsPP2C09 是脱落酸信号传导中的负调控因子，在平衡水稻植物生长和耐旱性方面发挥着重要作用
DOI：
10.1111/nph.16670
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发表时间：
2020-07-09
期刊：
影响因子：
9.4
通讯作者：
Zhou, Yong
中科院分区：
文献类型：
--
作者：
Miao, Jun;Li, Xianfeng;Zhou, Yong
植物在植物生长和抗逆性之间保持动态平衡，以优化其适应性并确保生存。本研究研究了a枝2C型蛋白磷酸酶（PP2C）编码基因OsPP2C09在水稻生长与耐旱性权衡调控中的作用。OsPP2C09蛋白与ABA信号的核心组分相互作用，并在体外显示PP2C磷酸酶活性。ospp2c09正向影响植物生长，但通过ABA信号负向调节植物抗旱性。外源ABA处理能快速诱导ospp2c09的转录和蛋白水平，抑制过量的ABA信号和植物生长停滞。正常条件下，ospp2c09在根中的转录水平远高于在茎中的转录水平。经ABA、聚乙二醇和脱水处理后，ospp2c09转录本在根中的积累速率大于在茎中的积累速率。在干旱胁迫条件下，根和芽之间的这种差异表达可能会增加植物的根冠比。该研究为OsPP2C09在协调植物生长和抗旱性中的作用提供了新的思路。特别是，我们认为ospp2c09介导的ABA脱敏有助于水稻在干旱胁迫条件下的根伸长。
Plants maintain a dynamic balance between plant growth and stress tolerance to optimise their fitness and ensure survival. Here, we investigated the roles of a clade A type 2C protein phosphatase (PP2C)-encoding gene,OsPP2C09, in regulating the trade-off between plant growth and drought tolerance in rice (Oryza sativaL.). The OsPP2C09 protein interacted with the core components of abscisic acid (ABA) signalling and showed PP2C phosphatase activityin vitro.OsPP2C09positively affected plant growth but acted as a negative regulator of drought tolerance through ABA signalling. Transcript and protein levels ofOsPP2C09were rapidly induced by exogenous ABA treatments, which suppressed excessive ABA signalling and plant growth arrest.OsPP2C09transcript levels in roots were much higher than those in shoots under normal conditions. After ABA, polyethylene glycol and dehydration treatments, the accumulation rate ofOsPP2C09transcripts in roots was more rapid and greater than that in shoots. This differential expression between the roots and shoots may increase the plant's root-to-shoot ratio under drought-stress conditions. This study sheds new light on the roles of OsPP2C09 in coordinating plant growth and drought tolerance. In particular, we propose that OsPP2C09-mediated ABA desensitisation contributes to root elongation under drought-stress conditions in rice.