Immunochemical and cytochemical detection of wall components of germinated pollen of gymnosperms
Immunochemical and cytochemical detection of wall components of germinated pollen of gymnosperms
复制标题
裸子植物萌发花粉壁成分的免疫化学和细胞化学检测
DOI：
10.1080/00173130260045468
复制
发表时间：
2002
期刊：
影响因子：
0.9
通讯作者：
N. Nakamura
中科院分区：
文献类型：
--
作者：
R. Yatomi;Sumio Nakamura;N. Nakamura
为了阐明裸子植物花粉的细胞壁结构，对6科8属14种裸子植物萌发花粉细胞壁成分的分布进行了细胞化学分析，采用4种多糖染色染料、4种多糖单克隆抗体和2种阿拉伯半乳聚糖蛋白（AGPs）单克隆抗体进行了免疫化学分析。花粉的内壁和管壁均含有AGPs和纤维素。石竹科、松科、红杉科和柏科（针叶科）的花粉壁成分虽然差异不大，但组成相似，在内壁和管壁都有较多的agp，管壁中很少有果胶。另一方面，苏铁花粉中含有丰富的果胶，银杏花粉内壁和管壁中都含有丰富的g -(1,3)(1,4)-葡聚糖。g - 1,3 -葡聚糖在6种松的管壁中普遍存在，但在管壁中存在差异。被检测的裸子植物花粉管的管壁都不像被子植物的花粉管，花粉管由两层组成，外层是果胶层，内层是胼胝质层。这些结果表明，裸子植物的花粉管壁的形成与被子植物的花粉管有显著的差异，其壁的组成可能反映了裸子植物之间的分类关系。
To elucidate wall construction of gymnosperm pollen, we studied the distributions of cell wall constituents in the germinated pollen of 14 species of gymnosperms in 8 genera and 6 families cytochemically with 4 staining dyes for polysaccharides, and immunochemically with 4 monoclonal antibodies for polysaccharides and 2 monoclonal antibodies for arabinogalactan proteins (AGPs). All intines and tube walls of the pollen examined contained AGPs and cellulose. Although having small differences, pollen of the Podocarpaceae, Pinaceae, Taxodiaceae, and Cupressaceae (Coniferales) had a similar composition of wall constituents: much AGPs occurred in both the intine and the tube wall, pectins were rare in the tube wall. On the other hand, in Cycas revoluta pollen, pectins were abundant and in Ginkgo biloba , g -(1,3)(1,4)-glucan was abundant in both the intine and the tube wall. The occurrence of g -1, 3-glucan in the intines was common to six Pinus species, but its occurrence in the tube wall differed among them. None of the gymnosperm pollen tubes examined had tube walls similar to those of angiosperm pollen, which consist of two layers, a pectic outer layer and a callosic inner layer. These results suggest that wall formation in gymnosperm pollen tubes is remarkably different from that of angiosperm pollen tubes and the composition of the wall constitutes may reflect a taxonomic relationship among gymnosperms.