Optimization Medium Composition for Vitamin K2 by Flavobacterium sp. using Response Surface Methodology and Addition of Arachis hypogaea
Optimization Medium Composition for Vitamin K2 by Flavobacterium sp. using Response Surface Methodology and Addition of Arachis hypogaea
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DOI：
10.1590/1678-4324-2016150343
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发表时间：
2016
影响因子：
1
通讯作者：
M. Tan;Hui Liu;Zhe-min Li;Xiaowen Sun;Zhiming Zheng;Genhai Zhao
中科院分区：
文献类型：
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作者：
M. Tan;Hui Liu;Zhe-min Li;Xiaowen Sun;Zhiming Zheng;Genhai Zhao
本研究的目的是通过发酵优化和添加花生来提高黄杆菌中维生素K2的产量。突变体SP-L-01。优化的培养条件为：摇瓶培养6d，初始pH值7.0±0.2，温度37℃，摇床转速120r/min，装液量30mL，接种量2%。经响应面法优化后，发酵基组成为麦芽糖23.8g/L、葡萄糖9.69g/L、牛肉膏15g/L、K2HPO44.5g/L、氯化钠3.0g/L、硫酸镁0.3g/L，维生素K2产量达10.97 mg/L，比优化前(6.12 mg/L)提高了79.25%。摇片培养72h，添加3 mg/mL花生，可使菜籽酮-4(MK4)产量提高371%，菜籽酮-6(MK6)产量提高149%。将花生的主要成分D-(+)-儿茶素单独添加到培养基中，也能促进维生素K2的合成。
The purpose of this research was to enhance the production of vitamin K2 by fermentation optimization and Arachis hypogaea supplementation in Flavobacterium sp. mutant SP-L-01. Optimized culture condition were as follows: 6-days shake-flask culture at 37oC with initial pH value 7.0 ± 0.2, shaking speed in 120 r/min and medium volume of 30 mL with 2% inoculums. After optimization of fermentation medium by response surface methodology (RSM), optimized medium were maltose 23.8 g/l, glucose 9.69 g/l, beef extract 15 g/l, K2HPO4 4.5 g/l，NaCl 3.0 g/l and MgSO4·7H2O 0.3 g/l. Production of vitamin K2 after optimization reached to 10.97 mg/l, which is 79.25% higher than that before optimization (6.12 mg/l). 3 mg/mL of arachis hypogaea was added into the medium at 72 h of shake-flake cultivation, which improved the production of menaquinone-4 (MK4) up to 371% and menaquinone-6 (MK6) up to 149% higher than those of the original medium. D-(+)-catechin, one of the components of arachis hypogaea, was added alone into the medium, which also improved the vitamin K2 synthesis.