Irreversible Site-Specific Hydrazinolysis of Proteins by Use of Sortase
Irreversible Site-Specific Hydrazinolysis of Proteins by Use of Sortase
复制标题
使用分选酶对蛋白质进行不可逆位点特异性肼解
DOI：
10.1002/anie.201310010
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发表时间：
2014-02-17
影响因子：
16.6
通讯作者：
Liu, Lei
中科院分区：
文献类型：
--
作者：
Li, Yi-Ming;Li, Yi-Tong;Liu, Lei
sortase介导的蛋白质与肼或其衍生物的肼解用于生产重组蛋白肼。该方法通过使用重组蛋白酰肼作为硫酯替代物为蛋白质半合成提供了一种替代方法。它还为具有功能单元的蛋白质的c端修饰以及C-to-C融合蛋白的制备提供了一种替代方法。
Sortase-mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production of recombinant protein hydrazides. This process provides an alternative approach for protein semisynthesis through the use of recombinant protein hydrazides as thioester surrogates. It also provides an alternative method for C-terminal modification of proteins with functional units as well as for the preparation of C-to-C fusion proteins.