Role of muscarinic cholinergic receptors in regulation of guanosine 3':5'-cyclic monophosphate content in mammalian brain, heart muscle, and intestinal smooth muscle.
Role of muscarinic cholinergic receptors in regulation of guanosine 3':5'-cyclic monophosphate content in mammalian brain, heart muscle, and intestinal smooth muscle.
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毒蕈碱胆碱能受体在调节哺乳动物脑、心肌和肠平滑肌中鸟苷 3:5-环单磷酸含量中的作用。
DOI：
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发表时间：
1972
影响因子：
11.1
通讯作者：
P. Greengard
中科院分区：
文献类型：
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作者：
T. P. Lee;J. Kuo;P. Greengard
已经对哺乳动物大脑皮层、心室和回肠切片中鸟苷 3':5'-环单磷酸（环 GMP）和腺苷 3':5'-环单磷酸（环 AMP）的浓度研究了乙酰胆碱以及模拟或阻断其生理作用的药物的作用。在所有研究的三种组织中，乙酰胆碱和主要具有毒蕈碱作用的拟胆碱药物，例如乙酰胆碱、氨甲酰甲胆碱和毛果芸香碱，可诱导环 GMP 浓度增加，而环 AMP 浓度没有变化或略有下降。另一方面，四甲基铵是一种主要具有烟碱作用的拟胆碱剂，不会显着改变环 GMP 或环 AMP 的浓度。乙酰胆碱及其毒蕈碱类似物诱导的环鸟苷酸组织含量的增加被毒蕈碱阻断剂阿托品拮抗，但烟碱阻断剂六甲铵则不能拮抗。这些和其他结果表明乙酰胆碱与毒蕈碱受体而不是与烟碱受体的相互作用导致环GMP浓度增加。这些数据与环 GMP 可能介导乙酰胆碱的毒蕈碱作用的假设相一致。胆碱能药物和肾上腺素能药物对肠平滑肌和心肌收缩生理学的拮抗作用反映在这些化合物对这些组织中环GMP和环AMP水平的拮抗作用中，并且可能是由这些化合物引起的。
Effects of acetylcholine and of agents that mimic or block its physiological actions have been studied upon concentrations of guanosine 3':5'-cyclic monophosphate (cyclic GMP) and adenosine 3':5'-cyclic monophosphate (cyclic AMP) in slices of mammalian cerebral cortex, heart ventricle, and ileum. Acetylcholine, and cholinomimetic agents with a predominantly muscarinic action, such as methacholine, bethanechol, and pilocarpine, induced an increase in the concentration of cyclic GMP, accompanied by no change or a slight decrease in the concentration of cyclic AMP, in all three tissues studied. Tetramethylammonium, a cholinomimetic agent with a predominantly nicotinic action, on the other hand, did not significantly alter concentrations of either cyclic GMP or cyclic AMP. The increase in the tissue content of cyclic GMP induced by acetylcholine and its muscarinic analogs was antagonized by atropine, a muscarinic blocking agent, but not by hexamethonium, a nicotinic blocking agent. These and other results suggest the generalization that the interaction of acetylcholine with muscarinic receptors, but not with nicotinic receptors, causes an increase in the concentration of cyclic GMP. The data are compatible with the hypothesis that cyclic GMP may mediate the muscarinic actions of acetylcholine. The antagonistic actions of cholinergic and adrenergic agents on the physiology of contraction of intestinal smooth muscle and of cardiac muscle are reflected in, and may result from, antagonistic actions of these compounds on the levels of both cyclic GMP and cyclic AMP in these tissues.