RECLASSIFICATION OF AMERICAN RHIZOBIUM-LEGUMINOSARUM BIOVAR PHASEOLI TYPE-I STRAINS AS RHIZOBIUM-ETLI SP-NOV
RECLASSIFICATION OF AMERICAN RHIZOBIUM-LEGUMINOSARUM BIOVAR PHASEOLI TYPE-I STRAINS AS RHIZOBIUM-ETLI SP-NOV
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DOI：
10.1099/00207713-43-2-374
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发表时间：
1993-04-01
期刊：
影响因子：
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通讯作者：
MARTINEZROMERO, E
中科院分区：
文献类型：
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作者：
SEGOVIA, L;YOUNG, JPW;MARTINEZROMERO, E
菜豆根瘤菌一新种。是基于16S核糖体DNA的序列分析提出的。该分类群，Rhizobium etli sp. nov.，先前被命名为豆科根瘤菌生物变种菜豆（Rhizobium leguminosarum biovar phaseoli）（I型菌株），其特征在于与豆类植物建立有效共生的能力、固氮酶结构基因的重复、共同的固氮酶基因组织成带有PsA和PsBC的两个独立的转录单位、多糖抑制基因psi和16S核糖体DNA序列的存在。对16SrRNA基因的一个片段的序列分析表明，该基因与R.豆科植物此外，所有R. ETLI菌株具有相同的序列。我们描述了这些分析，并讨论了支持我们建议的其他证据。
A new Rhizobium species that nodulates Phaseolus vulgaris L. is proposed on the basis of a sequence analysis of 16S ribosomal DNA. This taxon, Rhizobium etli sp. nov., was previously named Rhizobium leguminosarum biovar phaseoli (type I strains) and is characterized by the capacity to establish an effective symbiosis with bean plants, the reiteration of the nitrogenase structural genes, the organization of the common nodulation genes into two separate transcriptional units bearing nodA and nodBC, the presence of the polysaccharide inhibition gene, psi, and the 16S ribosomal DNA sequence. An analysis of the sequence of a fragment of the 16S rRNA gene shows that this gene is quite different from the gene of R. leguminosarum. In addition, all R. etli strains have identical sequences. We describe these analyses and discuss additional evidence supporting our proposal.