Probiotic Enterococcus faecalis Symbioflor 1 ameliorates pathobiont-induced miscarriage through bacterial antagonism and Th1-Th2 modulation in pregnant mice
Probiotic Enterococcus faecalis Symbioflor 1 ameliorates pathobiont-induced miscarriage through bacterial antagonism and Th1-Th2 modulation in pregnant mice
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益生菌粪肠球菌 Symbioflor 1 通过细菌拮抗和 Th1-Th2 调节改善怀孕小鼠中病原体引起的流产
DOI：
10.1007/s00253-020-10609-9
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发表时间：
2020-04-20
影响因子：
5
通讯作者：
Wei, Hua
中科院分区：
文献类型：
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作者：
Tao, Yue;Huang, Fuqing;Wei, Hua
致病性和益生菌性肠球菌之间的菌 - 菌相互作用以及肠球菌与肠上皮之间的菌 - 宿主相互作用日益受到关注，但这些相互作用对宿主妊娠的影响在很大程度上仍未被探索。在本研究中，我们评估了益生菌屎肠球菌Symbioflor 1或/和致病性屎肠球菌OG1RF对怀孕小鼠流产的影响。通过体外竞争、排斥和置换试验，观察到屎肠球菌Symbioflor 1对屎肠球菌OG1RF的拮抗特性，并且前者在体内抑制了后者的移位。通过屎肠球菌Symbioflor 1干预，屎肠球菌OG1RF攻击诱导的流产率显著降低了28%；并且这种益生菌干预极大地改善了怀孕小鼠回肠、结肠、子宫和胎盘的组织完整性以及胎盘血细胞密度。与对照组相比，益生菌干预显著上调了白细胞介素 - 10（IL - 10）和转化生长因子 - β（TGF - β）的水平，下调了干扰素 - γ（IFN - γ）的水平，并提高了孕酮水平，扭转了妊娠早期不良妊娠结局所报道的Th1优势状态的趋势。总之，屎肠球菌Symbioflor 1减少了屎肠球菌OG1RF的移位，防止了病原体诱导的组织损伤，并在妊娠早期使Th1 - Th2平衡向Th2优势转变，从而降低了流产率。
The bacterium-bacterium interaction between pathogenic and probiotic Enterococcus as well as the bacterium-host interaction between Enterococcus and intestinal epithelium has drawn increasing attentions, but the influence of those interactions on host pregnancy remains largely unexplored. In the present study, we evaluated the effects of probiotic E. faecalis Symbioflor 1 or/and pathogenic E. faecalis OG1RF on the miscarriage of pregnant mice. Using in vitro assays of competition and exclusion and displacement, antagonistic property of E. faecalis Symbioflor 1 against E. faecalis OG1RF was observed, and the former inhibited the translocation of the later in vivo. The rate of miscarriage induced by E. faecalis OG1RF challenge was significantly reduced by 28% with E. faecalis Symbioflor 1 intervention; and the tissue integrity of ileum, colon, uterus, and placenta and placental blood cell density in pregnant mice were drastically improved by such probiotic intervention. Compared with the controls, probiotic intervention significantly upregulated the level of IL-10 and TGF-β, downregulated levels of IFN-γ, and increased progesterone level that reversed the trend of being Th1 predominance state reported for adverse pregnancy outcome at early pregnancy stage. In conclusion, E. faecalis Symbioflor 1 decreased the translocation of E. faecalis OG1RF, prevented pathogen-induced tissue damage, and changed Th1-Th2 homeostasis toward Th2 predominance during early pregnancy resulting in decreased miscarriage. •The mechanism of how probiotic E. faecalis Symbioflor 1 improves pregnancy of mice • Influence of interactions of pathogenic and probiotic Enterococcus on host pregnancy • E. faecalis Symbioflor 1 change Th1-Th2 homeostasis toward Th2 predominance